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Hörner, M.* ; Becker, J.* ; Bohnert, R.* ; Baños, M.* ; Jerez-Longres, C.* ; Mühlhäuser, V.* ; Härrer, D.* ; Wong, T.W. ; Meier, M. ; Weber, W.*

A photoreceptor-based hydrogel with red light-responsive reversible sol-gel transition as transient cellular matrix.

Adv. Mater. Technol. 8, 10:2300195 (2023)
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Open Access Hybrid
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Hydrogels with adjustable mechanical properties have been engineered as matrices for mammalian cells and allow the dynamic, mechano-responsive manipulation of cell fate and function. Recent research yields hydrogels, where biological photoreceptors translated optical signals into a reversible and adjustable change in hydrogel mechanics. While their initial application provides important insights into mechanobiology, broader implementation is limited by a small dynamic range of addressable stiffness. Herein, this limitation is overcome by developing a photoreceptor-based hydrogel with reversibly adjustable stiffness from ≈800 Pa to the sol state. The hydrogel is based on star-shaped polyethylene glycol, functionalized with the red/far-red light photoreceptor phytochrome B (PhyB), or phytochrome-interacting factor 6 (PIF6). Upon illumination with red light, PhyB heterodimerizes with PIF6, thus crosslinking the polymers and resulting in gelation. However, upon illumination with far-red light, the proteins dissociate and trigger a complete gel-to-sol transition. The hydrogel's light-responsive mechanical properties are comprehensively characterized and it is applied as a reversible extracellular matrix for the spatiotemporally controlled deposition of mammalian cells within a microfluidic chip. It is anticipated that this technology will open new avenues for the site- and time-specific positioning of cells and will contribute to overcome spatial restrictions.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Cell Deposition ; Cellular Matrix ; Hydrogels ; Materials ; Microfluidics ; Optogenetics; In-vivo; Wavelength; Culture; Growth
Sprache englisch
Veröffentlichungsjahr 2023
HGF-Berichtsjahr 2023
ISSN (print) / ISBN 2365-709X
e-ISSN 2365-709X
Zeitschrift Advanced Materials Technologies
Quellenangaben Band: 8, Heft: 16, Seiten: 10, Artikelnummer: 2300195 Supplement: ,
Verlag Wiley
Verlagsort 111 River St, Hoboken, Nj 07030 Usa
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Pioneer Campus (HPC)
POF Topic(s) 30201 - Metabolic Health
Forschungsfeld(er) Pioneer Campus
PSP-Element(e) G-510002-001
Förderungen European Research Council (ERC)
Projekt DEAL
Ministry for Science, Research and Arts of the state of Baden-Wurttemberg
Excellence Initiative of the German Federal and State Governments - BIOSS
German Research Foundation (Deutsche Forschungsgemeinschaft, DFG) under Germany's Excellence Strategy - CIBSS
European Union (ERC)
DOI 10.1002/admt.202300195
WOS ID 001010000400001
Scopus ID 85162054017
Erfassungsdatum 2023-12-08
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