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Sinreih, M.* ; Gjorgoska, M.* ; Möller, G. ; Adamski, J. ; Rizner, T.L.*

17β-Hydroxysteroid dehydrogenases types 1 and 2: Enzymatic assays based on radiometric and mass-spectrometric detection.

Methods Enzymol. 689, 201-234 (2023)
DOI PMC
The 17β-hydroxysteroid dehydrogenase type 1 (HSD17B1) has a key role in estrogen biosynthesis as it catalyzes the reduction of estrone to the most potent estrogen, estradiol. Estradiol has a high affinity for estrogen receptors and thus stimulates their transactivation, which leads to cell proliferation and numerous other effects. HSD17B2 catalyzes the oxidation of estradiol to the less potent estrone, thereby decreasing estrogen receptor activation, which results in reduction of estrogen-associated effects. HSD17B1 and HSD17B2 overexpressing E.coli homogenates or recombinant enzymes can be used for screening and development of drugs against various pathologies such as cancer, endometriosis or osteoporosis. Here we describe the preparation of HSD17B1 and HSD17B2 bacterial homogenates and purified recombinant HSD17B1 protein as enzyme sources as well as enzymatic assays based on radiometric and mass-spectrometric detection for enzyme characterization.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter E. Coli Overexpression ; Hsd17b1 ; Hsd17b2 ; Kinetic Characterization ; Recombinant Protein; Human Estrogenic 17-beta-hydroxysteroid-dehydrogenase; Placental Estradiol-17-beta Dehydrogenase; Expression; Inhibitors; Derivatives; Kinetics; Enzymes; Cancer; Tissue
Sprache englisch
Veröffentlichungsjahr 2023
HGF-Berichtsjahr 2023
ISSN (print) / ISBN 0076-6879
e-ISSN 0076-6879
Zeitschrift Methods in Enzymology
Quellenangaben Band: 689, Heft: , Seiten: 201-234 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort 525 B Street, Suite 1900, San Diego, Ca 92101-4495 Usa
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Diabetes and Cancer (IDC)
Institute of Experimental Genetics (IEG)
POF Topic(s) 30201 - Metabolic Health
Forschungsfeld(er) Helmholtz Diabetes Center
Genetics and Epidemiology
PSP-Element(e) G-502594-001
G-500600-001
Förderungen Slovenian Research Agency
DOI 10.1016/bs.mie.2023.04.015
WOS ID 001101778800008
Scopus ID 85160077390
PubMed ID 37802571
Erfassungsdatum 2023-10-18
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