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Song, S.* ; van Dijk, F.* ; Vasse, G.F.* ; Liu, Q. ; Gosselink, I.F.* ; Weltjens, E.* ; Remels, A.H.* ; de Jager, M.H.* ; Bos, S.* ; Li, C. ; Stöger, T. ; Rehberg, M. ; Kutschke, D. ; van Eck, G.* ; Wu, X.* ; Willems, S.H.* ; Boom, D.* ; Kooter, I.M.* ; Spierings, D.* ; Wardenaar, R.* ; Cole, M.* ; Nawijn, M.C.* ; Salvati, A.* ; Gosens, R.* ; Melgert, B.N.*

Inhalable textile microplastic fibers impair airway epithelial differentiation.

Am. J. Respir. Crit. Care Med. 209, 427-443 (2024)
Verlagsversion Postprint DOI PMC
Open Access Green
RATIONALE: Microplastics are a pressing global concern and inhalation of microplastic fibers has been associated with interstitial and bronchial inflammation in flock workers. However, how microplastic fibers affect the lungs is unknown. OBJECTIVES: Our aim was to assess the effects of 12x31 µm nylon 6,6 (nylon) and 15x52 µm polyethylene terephthalate (polyester) textile microplastic fibers on lung epithelial growth and differentiation. METHODS: We used human and murine alveolar and airway-type organoids as well as air-liquid interface cultures derived from primary lung epithelial progenitor cells and incubated these with either nylon or polyester fibers or nylon leachate. In addition, mice received one dose of nylon fibers or nylon leachate and 7 days later organoid-forming capacity of isolated epithelial cells was investigated. MEASUREMENTS AND MAIN RESULTS: We observed that nylon microfibers, more than polyester, inhibited developing airway organoids and not established ones. This effect was mediated by components leaching from nylon. Epithelial cells isolated from mice exposed to nylon fibers or leachate, also formed fewer airway organoids, suggesting long-lasting effects of nylon components on epithelial cells. Part of these effects were recapitulated in human air-liquid interface cultures. Transcriptome analysis revealed upregulation of Hoxa5 post-exposure to nylon fibers. Inhibiting Hoxa5 during nylon exposure restored airway organoid formation, confirming Hoxa5's pivotal role in the effects of nylon. CONCLUSIONS: These results suggest that components leaching from nylon 6,6 may especially harm developing airways and/or airways undergoing repair and we strongly encourage to characterize both hazard of and exposure to microplastic fibers in more detail.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Nylon ; Polyethylene Terephthalate ; Airway Organoids ; Lung Epithelial Repair ; Polyester; Mesenchymal Transition; Lung; Beta; Rats
Sprache englisch
Veröffentlichungsjahr 2024
Prepublished im Jahr 2023
HGF-Berichtsjahr 2023
ISSN (print) / ISBN 1073-449X
e-ISSN 1535-4970
Zeitschrift American Journal of Respiratory and Critical Care Medicine
Quellenangaben Band: 209, Heft: 4, Seiten: 427-443 Artikelnummer: , Supplement: ,
Verlag American Thoracic Society
Verlagsort 25 Broadway, 18 Fl, New York, Ny 10004 Usa
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Lung Health and Immunity (LHI)
POF Topic(s) 30202 - Environmental Health
Forschungsfeld(er) Lung Research
PSP-Element(e) G-505000-001
Förderungen ZonMw
Microplastics and Health
ZonMw/Health Holland consortium grant MOMENTUM
DOI 10.1164/rccm.202211-2099OC
WOS ID 001193268600014
Scopus ID 85185219245
PubMed ID 37971785
Erfassungsdatum 2023-11-28
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