PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Marder, M. ; Remmert, C. ; Perschel, J.A. ; Otgonbayar, M. ; von Toerne, C. ; Hauck, S.M. ; Bushe, J. ; Feuchtinger, A. ; Sheikh, B. ; Moussus, M. ; Meier, M.

Stem cell-derived vessels-on-chip for cardiovascular disease modeling.

Cell Rep. 43:114008 (2024)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
The metabolic syndrome is accompanied by vascular complications. Human in vitro disease models are hence required to better understand vascular dysfunctions and guide clinical therapies. Here, we engineered an open microfluidic vessel-on-chip platform that integrates human pluripotent stem cell-derived endothelial cells (SC-ECs). The open microfluidic design enables seamless integration with state-of-the-art analytical technologies, including single-cell RNA sequencing, proteomics by mass spectrometry, and high-resolution imaging. Beyond previous systems, we report SC-EC maturation by means of barrier formation, arterial toning, and high nitric oxide synthesis levels under gravity-driven flow. Functionally, we corroborate the hallmarks of early-onset atherosclerosis with low sample volumes and cell numbers under flow conditions by determining proteome and secretome changes in SC-ECs stimulated with oxidized low-density lipoprotein and free fatty acids. More broadly, our organ-on-chip platform enables the modeling of patient-specific human endothelial tissue and has the potential to become a general tool for animal-free vascular research.
Impact Factor
Scopus SNIP
Altmetric
7.500
0.000
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Cp: Stem Cell Research ; Atherosclerosis ; Disease Modeling ; Hipsc-derived Endothelial Cells ; Open Microfluidics ; Single-cell Sequencing ; Vessel-on-chip; Nitric-oxide Synthase; Shear-stress; Enrichment Analysis; Endothelial-cells; Web Server; Vitronectin; Expression; Generation; Platform
Sprache englisch
Veröffentlichungsjahr 2024
HGF-Berichtsjahr 2024
ISSN (print) / ISBN 2211-1247
e-ISSN 2211-1247
Zeitschrift Cell Reports
Quellenangaben Band: 43, Heft: 4, Seiten: , Artikelnummer: 114008 Supplement: ,
Verlag Cell Press
Verlagsort 50 Hampshire St, Floor 5, Cambridge, Ma 02139 Usa
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Pioneer Campus (HPC)
CF Metabolomics & Proteomics (CF-MPC)
CF Pathology & Tissue Analytics (CF-PTA)
Helmholtz Institute for Metabolism, Obesity and Vascular Research (HI-MAG)
POF Topic(s) 30201 - Metabolic Health
30203 - Molecular Targets and Therapies
30202 - Environmental Health
Forschungsfeld(er) Pioneer Campus
Enabling and Novel Technologies

Helmholtz Diabetes Center
PSP-Element(e) G-510002-001
G-505700-001
A-630600-001
G-555000-001
A-630700-001
Förderungen ERC Consolidator Grant
EIslet project of the BMBF
Helmholtz Pioneer Campus
DOI 10.1016/j.celrep.2024.114008
WOS ID 001215439700001
Scopus ID 85188672222
PubMed ID 38536819
Erfassungsdatum 2024-05-21
[➜Korrektur melden]