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Duda, H.C.* ; von Toerne, C. ; Korbonits, L.* ; Didier, A.* ; Scholz, A.M.* ; Märtlbauer, E.* ; Hauck, S.M. ; Deeg, C.A.*

Cathepsin S is more abundant in serum of Mycobacterium avium subsp. paratuberculosis-Infected dairy cows.

Metabolites 14:215 (2024)
Verlagsversion DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of bovine paratuberculosis, a chronic granulomatous enteritis leading to economic losses and posing a risk to human health due to its zoonotic potential. The pathogen cannot reliably be detected by standard methods, and immunological procedures during the infection are not well understood. Therefore, the aim of our study was to explore host-pathogen interactions in MAP-infected dairy cows and to improve diagnostic tests. Serum proteomics analysis using quantitative label-free LC-MS/MS revealed 60 differentially abundant proteins in MAP-infected dairy cows compared to healthy controls from the same infected herd and 90 differentially abundant proteins in comparison to another control group from an uninfected herd. Pathway enrichment analysis provided new insights into the immune response to MAP and susceptibility to the infection. Furthermore, we found a higher abundance of Cathepsin S (CTSS) in the serum of MAP-infected dairy cows, which is involved in multiple enriched pathways associated with the immune system. Confirmed with Western blotting, we identified CTSS as a potential biomarker for bovine paratuberculosis. This study enabled a better understanding of procedures in the host-pathogen response to MAP and improved detection of paratuberculosis-diseased cattle.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Cathepsin S ; Mycobacterium Avium Subsp. Paratuberculosis ; Bovine ; Host–pathogen Response ; Immune System ; Quantitative Label-free Lc-ms/ms ; Serum Proteomics; Class-ii Molecules; Mhc-class-ii; Phagosomal Maturation; Bovine Macrophages; Invariant Chain; Expression; Diagnosis; Survival; Disease; Plasma
Sprache englisch
Veröffentlichungsjahr 2024
HGF-Berichtsjahr 2024
ISSN (print) / ISBN 2218-1989
e-ISSN 2218-1989
Zeitschrift Metabolites
Quellenangaben Band: 14, Heft: 4, Seiten: , Artikelnummer: 215 Supplement: ,
Verlag MDPI
Verlagsort St Alban-anlage 66, Ch-4052 Basel, Switzerland
Begutachtungsstatus Peer reviewed
Institut(e) CF Metabolomics & Proteomics (CF-MPC)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-505700-001
A-630700-001
Förderungen German Federal Ministry of Economics and Technology (via AiF)
DOI 10.3390/metabo14040215
WOS ID 001211154600001
Scopus ID 85191761574
PubMed ID 38668343
Erfassungsdatum 2024-06-10
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