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Pepin, A.-S. ; Schneider, R.

Emerging toolkits for decoding the co-occurrence of modified histones and chromatin proteins.

EMBO Rep. 25, 3202-3220 (2024)
Verlagsversion DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
In eukaryotes, DNA is packaged into chromatin with the help of highly conserved histone proteins. Together with DNA-binding proteins, posttranslational modifications (PTMs) on these histones play crucial roles in regulating genome function, cell fate determination, inheritance of acquired traits, cellular states, and diseases. While most studies have focused on individual DNA-binding proteins, chromatin proteins, or histone PTMs in bulk cell populations, such chromatin features co-occur and potentially act cooperatively to accomplish specific functions in a given cell. This review discusses state-of-the-art techniques for the simultaneous profiling of multiple chromatin features in low-input samples and single cells, focusing on histone PTMs, DNA-binding, and chromatin proteins. We cover the origins of the currently available toolkits, compare and contrast their characteristic features, and discuss challenges and perspectives for future applications. Studying the co-occurrence of histone PTMs, DNA-binding proteins, and chromatin proteins in single cells will be central for a better understanding of the biological relevance of combinatorial chromatin features, their impact on genomic output, and cellular heterogeneity.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Review
Schlagwörter Chromatin States ; Combinatorial Occurrence ; Histone Posttranslational Modifications ; Nonhistone Chromatin Proteins ; Single-cell Omics; Dna Interactions Invivo; Rna Polymerase-ii; Genome-wide Maps; Chip-seq; Posttranslational Modifications; Active Promoters; Tn5 Transposase; Cross-linking; Formaldehyde; Identification
Sprache englisch
Veröffentlichungsjahr 2024
HGF-Berichtsjahr 2024
ISSN (print) / ISBN 1469-221X
e-ISSN 1469-3178
Zeitschrift EMBO Reports
Quellenangaben Band: 25, Heft: 8, Seiten: 3202-3220 Artikelnummer: , Supplement: ,
Verlag EMBO Press
Verlagsort Campus, 4 Crinan St, London, N1 9xw, England
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Functional Epigenetics (IFE)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Helmholtz Diabetes Center
PSP-Element(e) G-502800-001
Förderungen Helmholtz Gemeinschaft
Deutsche Forschungsgemeinschaft (DFG, German Research foundation)
DOI 10.1038/s44319-024-00199-2
WOS ID 001282676400001
Scopus ID 85200975664
PubMed ID 39095610
Erfassungsdatum 2024-09-17
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