PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Meyer, M. ; Ortiz, O. ; Hrabě de Angelis, M. ; Wurst, W. ; Kühn, R.

Modeling disease mutations by gene targeting in one-cell mouse embryos.

Proc. Natl. Acad. Sci. U.S.A. 109, 9354-9359 (2012)
Verlagsversion Volltext DOI PMC
Open Access Gold
Gene targeting by zinc-finger nucleases in one-cell embryos provides an expedite mutagenesis approach in mice, rats, and rabbits. This technology has been recently used to create knockout and knockin mutants through the deletion or insertion of nucleotides. Here we apply zinc-finger nucleases in one-cell mouse embryos to generate disease-related mutants harboring single nucleotide or codon replacements. Using a gene-targeting vector or a synthetic oligodesoxynucleotide as template for homologous recombination, we introduced missense and silent mutations into the Rab38 gene, encoding a small GTPase that regulates intracellular vesicle trafficking. These results demonstrate the feasibility of seamless gene editing in one-cell embryos to create genetic disease models and establish synthetic oligodesoxynucleotides as a simplified mutagenesis tool.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Altmetric
9.681
0.807
45
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter ZINC-FINGER NUCLEASES; HERMANSKY-PUDLAK-SYNDROME; KNOCKOUT RATS; GENOME MANIPULATION; PROTEIN-TRAFFICKING; MICE; RAB38; MICROINJECTION; BIOGENESIS; DISRUPTION
Sprache
Veröffentlichungsjahr 2012
HGF-Berichtsjahr 2012
ISSN (print) / ISBN 0027-8424
e-ISSN 1091-6490
Zeitschrift Proceedings of the National Academy of Sciences of the United States of America
Quellenangaben Band: 109, Heft: 24, Seiten: 9354-9359 Artikelnummer: , Supplement: ,
Verlag National Academy of Sciences
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Developmental Genetics (IDG)
Institute of Experimental Genetics (IEG)
POF Topic(s) 30204 - Cell Programming and Repair
30201 - Metabolic Health
Forschungsfeld(er) Genetics and Epidemiology
PSP-Element(e) G-500500-001
G-500600-003
PubMed ID 22660928
DOI 10.1073/pnas.1121203109
WOS ID WOS:000305511300036
Scopus ID 84862184780
Erfassungsdatum 2012-06-06
[➜Korrektur melden]