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Hochstatter, J.* ; Hölzel, M. ; Rohrmoser, M. ; Schermelleh, L.* ; Leonhardt, H.* ; Keough, R.* ; Gonda, T.J.* ; Imhof, A.* ; Eick, D. ; Längst, G.* ; Németh, A.*

Myb-binding protein 1a (Mybbp1a) regulates levels and processing of pre-ribosomal RNA.

J. Biol. Chem. 287, 24365-24377 (2012)
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Ribosomal RNA gene transcription, co-transcriptional processing, and ribosome biogenesis are highly coordinated processes that are tightly regulated during cell growth. In this study we discovered that Mybbp1a is associated with both the RNA polymerase I complex and the ribosome biogenesis machinery. Using a reporter assay that uncouples transcription and RNA processing, we show that Mybbp1a represses rRNA gene transcription. In addition, overexpression of the protein reduces RNA polymerase I loading on endogenous rRNA genes as revealed by chromatin immunoprecipitation experiments. Accordingly, depletion of Mybbp1a results in an accumulation of the rRNA precursor in vivo but surprisingly also causes growth arrest of the cells. This effect can be explained by the observation that the modulation of Mybbp1a protein levels results in defects in pre-rRNA processing within the cell. Therefore, the protein may play a dual role in the rRNA metabolism, potentially linking and coordinating ribosomal DNA transcription and pre-rRNA processing to allow for the efficient synthesis of ribosomes.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Gene Regulation; Nucleolus; Ribosomal RNA (rRNA); Ribosomal RNA Processing; Transcription Regulation; POLYMERASE-I TRANSCRIPTION; FACTOR TIF-IA; NUCLEAR ANTIGEN 1; SACCHAROMYCES-CEREVISIAE; NUCLEOLAR PROTEIN; MOLECULAR-CLONING; GENE-EXPRESSION; DEPENDENT TRANSCRIPTION; YEAST; CHROMATIN
Sprache englisch
Veröffentlichungsjahr 2012
HGF-Berichtsjahr 2012
ISSN (print) / ISBN 0021-9258
e-ISSN 1083-351X
Zeitschrift Journal of Biological Chemistry, The
Quellenangaben Band: 287, Heft: 29, Seiten: 24365-24377 Artikelnummer: , Supplement: ,
Verlag American Society for Biochemistry and Molecular Biology
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Clinical Molecular Biology and Tumor Genetics (K.MOLBI)
POF Topic(s) 30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Forschungsfeld(er) Immune Response and Infection
PSP-Element(e) G-501490-001
PubMed ID 22645127
DOI 10.1074/jbc.M111.303719
WOS ID WOS:000306651100031
Scopus ID 84863799516
Erfassungsdatum 2012-07-26
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