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Grimmig, B. ; Gonzalez-Perez, M.N. ; Leubner-Metzger, G.* ; Vögeli-Lange, R.* ; Meins, F. J.r.* ; Hain, R.* ; Penuelas, J.* ; Heidenreich, B. ; Langebartels, C. ; Ernst, D. ; Sandermann, H.

Ozone-induced gene expression occurs via ethylene-dependent and -independent signalling.

Plant Mol. Biol. 51, 599-607 (2003)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Recent studies suggest that ethylene is involved in signalling ozone-induced gene expression. We show here that application of ozone increased glucuronidase (GUS) expression of chimeric reporter genes regulated by the promoters of the tobacco class I beta-1,3-glucanases (GLB and Gln2) and the grapevine resveratrol synthase (Vst1) genes in transgenic tobacco leaves. 5'-deletion analysis of the class I beta-1,3-glucanase promoter revealed that ozone-induced gene regulation is mainly mediated by the distal enhancer region containing the positively acting ethylene-responsive element (ERE). In addition, application of 1-methylcyclopropene (1-MCP), an inhibitor of ethylene action, blocked ozone-induced class I beta-1,3-glucanase promoter activity. Enhancer activity and ethylene-responsiveness depended on the integrity of the GCC boxes, cis-acting elements present in the ERE of the class I beta-1,3-glucanase and the basic-type pathogenesis-related PR-1 protein (PRB-1b) gene promoters. The minimal PRB-1b promoter containing only the ERE with intact GCC boxes, was sufficient to confer 10-fold ozone inducibility to a GUS-reporter gene, while a substitution mutation in the GCC box abolished ozone responsiveness. The ERE region of the class I beta-1,3-glucanase promoter containing two intact GCC boxes confered strong ozone inducibility to a minimal cauliflower mosaic virus (CaMV) 35S RNA promoter, whereas two single-base substitution in the GCC boxes resulted in a complete loss of ozone inducibility. Taken together, these datastrongly suggest that ethylene is signalling ozone-induced expression of class I beta-l,3-glucanase and PRB-1b genes. Promoter analysis of the stilbene synthase Vst1 gene unravelled different regions for ozone and ethylene-responsiveness. Application of 1-MCP blocked ethylene-induced Vst1 induction, but ozone induction was not affected. This shows that ozone-induced gene expression occurs via at least two different signalling mechanisms and suggests an additional ethylene independent signalling pathway for ozone-induced expression of genes involved in phytoalexin biosynthesis.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter ethylene; gene regulation; ozone; pathogenesis-related; promoter; resveratrol; stilbene; BIOCHEMICAL-PLANT RESPONSES; RESVERATROL SYNTHASE PROMOTER; PATHOGENESIS-RELATED PROTEINS; PROGRAMMED CELL-DEATH; PINUS-SYLVESTRIS L.; SALICYLIC-ACID; SCOTS PINE; DISEASE RESISTANCE; STILBENE SYNTHASE; OXIDATIVE BURST
ISSN (print) / ISBN 0167-4412
e-ISSN 0167-4412
Zeitschrift Plant Molecular Biology
Quellenangaben Band: 51, Heft: 4, Seiten: 599-607 Artikelnummer: , Supplement: ,
Verlag Springer
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biochemical Plant Pathology (BIOP)