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Conditional brain-specific knockdown of MAPK using Cre/loxP regulated RNA interference.
Nucleic Acids Res. 35:e90 (2007)
In the last years, RNA interference (RNAi)-mediated gene knockdown has developed into a routine method to assess gene function in cultured mammalian cells in a fast and easy manner. For the use of this technique in developing or adult mice, short hairpin (sh)RNA vectors expressed stably from the genome are a faster alternative to conventional knockout approaches. Here we describe an advanced strategy for conditional gene knockdown in mice, where we used the Cre/loxP system to activate RNAi in a time and tissue dependent manner in the adult mouse brain. By placing conditional RNAi constructs into the defined genomic Rosa26 locus and by using recombinase mediated cassette exchange (RMCE) instead of laborious homologous recombination, we developed a fast, easy and reproducible approach to assess gene function in adult mice. We applied this technique to three genes of the MAPK signaling pathway-Braf, Mek1 and Mek2-and demonstrate here the potential of this new tool in mouse mutagenesis.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Times Cited
Scopus
Cited By
Cited By
Altmetric
6.317
0.000
80
81
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
MAMMALIAN-CELLS; GENE-EXPRESSION; TRANSGENIC MICE; FLP RECOMBINATION; IN-VIVO; CRE; SYSTEM; MESSENGER; CASCADE; TRANSMISSION
Sprache
englisch
Veröffentlichungsjahr
2007
HGF-Berichtsjahr
0
ISSN (print) / ISBN
0305-1048
e-ISSN
1362-4962
Zeitschrift
Nucleic Acids Research
Quellenangaben
Band: 35,
Heft: 12,
Artikelnummer: e90
Verlag
Oxford University Press
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Developmental Genetics (IDG)
POF Topic(s)
30204 - Cell Programming and Repair
Forschungsfeld(er)
Genetics and Epidemiology
PSP-Element(e)
G-500500-001
PubMed ID
17586814
WOS ID
000247817700039
Scopus ID
34547629188
Erfassungsdatum
2007-12-04