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Webb, K.J. ; Norton, W.H.J. ; Trümbach, D. ; Meijer, A.H.* ; Ninkovic, J. ; Topp, S. ; Heck, D. ; Marr, C. ; Wurst, W. ; Theis, F.J. ; Spaink, H.P.* ; Bally-Cuif, L.

Zebrafish reward mutants reveal novel transcripts mediating the behavioral effects of amphetamine.

Genome Biol. 10:R81 (2009)
Verlagsversion Volltext DOI PMC
Open Access Gold
Addiction is a pathological dysregulation of the brain's reward systems, determined by several complex genetic pathways. The conditioned place preference test provides an evaluation of the effects of drugs in animal models, allowing the investigation of substances at a biologically relevant level with respect to reward. Our lab has previously reported the development of a reliable conditioned place preference paradigm for zebrafish. Here, this test was used to isolate a dominant N-ethyl-N-nitrosourea (ENU)-induced mutant, no addiction (nad(dne3256)), which fails to respond to amphetamine, and which we used as an entry point towards identifying the behaviorally relevant transcriptional response to amphetamine. Results: Through the combination of microarray experiments comparing the adult brain transcriptome of mutant and wild-type siblings under normal conditions, as well as their response to amphetamine, we identified genes that correlate with the mutants' altered conditioned place preference behavior. In addition to pathways classically involved in reward, this gene set shows a striking enrichment in transcription factor-encoding genes classically involved in brain development, which later appear to be reused within the adult brain. We selected a subset of them for validation by quantitative PCR and in situ hybridization, revealing that specific brain areas responding to the drug through these transcription factors include domains of ongoing adult neurogenesis. Finally, network construction revealed functional connections between several of these genes. Conclusions: Together, our results identify a new network of coordinated gene regulation that influences or accompanies amphetamine-triggered conditioned place preference behavior and that may underlie the susceptibility to addiction.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Review
Schlagwörter dendritic spine morphogenesis; gene-expression changes; neural stem-cells; adult hippocampal neurogenesis; conditioned place preference; lim-homeobox gene; drug-addiction; neuronal differentiation; synaptic plasticity; cholinergic neurons
Sprache englisch
Veröffentlichungsjahr 2009
HGF-Berichtsjahr 2009
ISSN (print) / ISBN 1474-760X
e-ISSN 1465-6906
Zeitschrift Genome Biology
Quellenangaben Band: 10, Heft: 7, Seiten: , Artikelnummer: R81 Supplement: ,
Verlag BioMed Central
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Developmental Genetics (IDG)
Institute of Bioinformatics and Systems Biology (IBIS)
POF Topic(s) 30501 - Systemic Analysis of Genetic and Environmental Factors that Impact Health
30204 - Cell Programming and Repair
30505 - New Technologies for Biomedical Discoveries
Forschungsfeld(er)
Genetics and Epidemiology
Enabling and Novel Technologies
PSP-Element(e) G-500100-001
G-500500-001
G-503700-001
PubMed ID 19646228
DOI 10.1186/gb-2009-10-7-r81
WOS ID WOS:000269309600016
Scopus ID 68849120041
Erfassungsdatum 2009-10-09
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