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Barjaktarovic, Z. ; Anastasov, N. ; Azimzadeh, O. ; Sriharshan, A. ; Sarioglu, H. ; Ueffing, M. ; Tammio, H.* ; Hakanen, A.* ; Leszczynski, D.* ; Atkinson, M.J. ; Tapio, S.

Integrative proteomic and microRNA analysis of primary human coronary artery endothelial cells exposed to low-dose gamma radiation.

Radiat. Environ. Biophys. 52, 87-98 (2013)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
High doses of ionising radiation significantly increase the risk of cardiovascular disease (CVD), the vascular endothelium representing one of the main targets. Whether radiation doses lower than 500 mGy induce cardiovascular damage is controversial. The aim of this study was to investigate radiation-induced expression changes on protein and microRNA (miRNA) level in primary human coronary artery endothelial cells after a single 200 mGy radiation dose (Co-60). Using a multiplex gel-based proteomics technology (2D-DIGE), we identified 28 deregulated proteins showing more than ±1.5-fold expression change in comparison with non-exposed cells. A great majority of the proteins showed up-regulation. Bioinformatics analysis indicated "cellular assembly and organisation, cellular function and maintenance and molecular transport" as the most significant radiation-responsive network. Caspase-3, a central regulator of this network, was confirmed to be up-regulated using immunoblotting. We also analysed radiation-induced alterations in the level of six miRNAs known to play a role either in CVD or in radiation response. The expression of miR-21 and miR-146b showed significant radiation-induced deregulation. Using miRNA target prediction, three proteins found differentially expressed in this study were identified as putative candidates for miR-21 regulation. A negative correlation was observed between miR-21 levels and the predicted target proteins, desmoglein 1, phosphoglucomutase and target of Myb protein. This study shows for the first time that a low-dose exposure has a significant impact on miRNA expression that is directly related to protein expression alterations. The data presented here may facilitate the discovery of low-dose biomarkers of radiation-induced cardiovascular damage.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Ionising Radiation ; Proteomics ; Low-dose Radiation ; Mir-21 ; Mir-146b ; Endothelium ; Radiation-induced Cardiovascular Disease; Ionizing-radiation ; Cardiovascular-disease ; Long-term ; Mortality Experience ; Polyacrylamide-gels ; Statistical-model ; Breast-cancer ; Heart-disease ; Follow-up ; Mayak Pa
Sprache englisch
Veröffentlichungsjahr 2013
HGF-Berichtsjahr 2013
ISSN (print) / ISBN 0301-634X
e-ISSN 1432-2099
Zeitschrift Radiation and Environmental Biophysics
Quellenangaben Band: 52, Heft: 1, Seiten: 87-98 Artikelnummer: , Supplement: ,
Verlag Springer
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Radiation Biology (ISB)
CF Metabolomics & Proteomics (CF-MPC)
Translational Metabolic Oncology (IDC-TMO)
POF Topic(s) 30202 - Environmental Health
30203 - Molecular Targets and Therapies
Forschungsfeld(er) Radiation Sciences
Enabling and Novel Technologies
PSP-Element(e) G-500200-001
G-505700-001
G-501000-001
PubMed ID 23138885
DOI 10.1007/s00411-012-0439-4
WOS ID WOS:000315385100009
Scopus ID 84874389322
Erfassungsdatum 2013-03-01
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