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Silencing of genes and alleles by RNAi in Anopheles gambiae.
Methods Mol. Biol. 923, 161-176 (2013)
Anopheles gambiae mosquitoes are the major vectors of human malaria parasites. However, mosquitoes are not passive hosts for parasites, actively limiting their development in vivo. Our current understanding of the mosquito antiparasitic response is mostly based on the phenotypic analysis of gene knockdowns obtained by RNA interference (RNAi), through the injection or transfection of long dsRNAs in adult mosquitoes or cultured cells, respectively. Recently, RNAi has been extended to silence specifically one allele of a given gene in a heterozygous context, thus allowing to compare the contribution of different alleles to a phenotype in the same genetic background.
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Anmerkungen
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Herausgeber
Menard, R.*
Schlagwörter
Malaria; Mosquito; RNAi; Reciprocal allele-specific RNAi; Real-time PCR; Western blot
Sprache
englisch
Veröffentlichungsjahr
2013
HGF-Berichtsjahr
0
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Konferenztitel
Malaria : Methods and Protocols
Zeitschrift
Methods in Molecular Biology
Quellenangaben
Band: 923,
Seiten: 161-176
Verlag
Springer
Verlagsort
Berlin [u.a.]
Begutachtungsstatus
Peer reviewed
Institut(e)
Research Unit Molecular Epidemiology (AME)
POF Topic(s)
30501 - Systemic Analysis of Genetic and Environmental Factors that Impact Health
Forschungsfeld(er)
Genetics and Epidemiology
PSP-Element(e)
G-504200-003
PubMed ID
22990777
Erfassungsdatum
2013-06-24