OpenSSL SSL_connect: Connection reset by peer in connection to v2.sherpa.ac.uk:443 PuSH - Publikationsserver des Helmholtz Zentrums München: Silencing of genes and alleles by RNAi in <em>Anopheles gambiae</em>.

PuSH - Publikationsserver des Helmholtz Zentrums München

Lamacchia, M.* ; Clayton, J.R.* ; Wang-Sattler, R. ; Steinmetz, L.M.* ; Levashina, E.A.* ; Blandin, S.A.*

Silencing of genes and alleles by RNAi in Anopheles gambiae.

Methods Mol. Biol. 923, 161-176 (2013)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Anopheles gambiae mosquitoes are the major vectors of human malaria parasites. However, mosquitoes are not passive hosts for parasites, actively limiting their development in vivo. Our current understanding of the mosquito antiparasitic response is mostly based on the phenotypic analysis of gene knockdowns obtained by RNA interference (RNAi), through the injection or transfection of long dsRNAs in adult mosquitoes or cultured cells, respectively. Recently, RNAi has been extended to silence specifically one allele of a given gene in a heterozygous context, thus allowing to compare the contribution of different alleles to a phenotype in the same genetic background.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Herausgeber Menard, R.*
Korrespondenzautor
Schlagwörter Malaria; Mosquito; RNAi; Reciprocal allele-specific RNAi; Real-time PCR; Western blot
ISSN (print) / ISBN 1064-3745
e-ISSN 1940-6029
Konferenztitel Malaria : Methods and Protocols
Zeitschrift Methods in Molecular Biology
Quellenangaben Band: 923, Heft: , Seiten: 161-176 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Berlin [u.a.]
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Molecular Epidemiology (AME)