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Lorenz, O.R.* ; Freiburger, L. ; Rutz, D.A.* ; Krause, M.* ; Zierer, B.K.* ; Alvira, S.* ; Cuéllar, J.* ; Valpuesta, J.M.* ; Madl, T. ; Sattler, M. ; Buchner, J.*

Modulation of the Hsp90 chaperone cycle by a stringent client protein.

Mol. Cell 53, 941-953 (2014)
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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Hsp90 is the most abundant molecular chaperone in the eukaryotic cell. One of the most stringent clients is the glucocorticoid receptor (GR), whose in vivo function strictly depends on the interaction with the Hsp90 machinery. However, the molecular mechanism of this interaction has been elusive. Here we have reconstituted the interaction of Hsp90 with hormone-bound GR using purified components. Our biochemical and structural analyses define the binding site for GR on Hsp90 and reveal that binding of GR modulates the conformational cycle of Hsp90. FRET experiments demonstrate that a partially closed form of the Hsp90 dimer is the preferred conformation for interaction. Consistent with this, the conformational cycle of Hsp90 is decelerated, and its ATPase activity decreases. Hsp90 cochaperones differentially affect formation of the Hsp90-GR complex, serving as control elements for cycle progression and revealing an intricate interplay of client and cochaperones as molecular modulators of the Hsp90 machine.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Heat-shock-protein; Ligand-binding Domain; Glucocorticoid-receptor; Steroid-receptor; Molecular Chaperone; Atpase Cycle; Conformational Dynamics; Crystal-structure; In-vivo; Escherichia-coli
Sprache englisch
Veröffentlichungsjahr 2014
HGF-Berichtsjahr 2014
ISSN (print) / ISBN 1097-2765
e-ISSN 1097-4164
Zeitschrift Molecular Cell
Quellenangaben Band: 53, Heft: 6, Seiten: 941-953 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Cambridge
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Structural Biology (STB)
POF Topic(s) 30203 - Molecular Targets and Therapies
30505 - New Technologies for Biomedical Discoveries
Forschungsfeld(er) Enabling and Novel Technologies
PSP-Element(e) G-503000-001
G-552800-001
PubMed ID 24613341
DOI 10.1016/j.molcel.2014.02.003
WOS ID WOS:000333329400010
Scopus ID 84896405318
Erfassungsdatum 2014-03-25
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