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Pertek, A. ; Meier, F. ; Irmler, M. ; Beckers, J. ; Skylaki, S. ; Endele, M. ; Wurst, W. ; Prakash, N. ; Kühn, R.

Simple derivation of transgene-free iPS cells by a dual recombinase approach.

Mol. Biotechnol. 56, 697-713 (2014)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Mammalian cells can be reprogrammed into induced pluripotent stem cells (iPSCs), a valuable tool for in vitro disease modeling and regenerative medicine. These applications demand for iPSCs devoid of reprogramming factor transgenes, but current procedures for the derivation of transgene-free iPSCs are inefficient and cumbersome. Here, we describe a new approach for the simple derivation of transgene-free iPSCs by the sequential use of two DNA recombinases, C31 Integrase and Cre, to control the genomic insertion and excision of a single, non-viral reprogramming vector. We show that such transgene-free iPSCs exhibit gene expression profiles and pluripotent developmental potential comparable to genuine, blastocyst-derived embryonic stem cells. As shown by a reporter iPSC line for the differentiation into midbrain dopaminergic neurons, the dual recombinase approach offers a simple and efficient way to derive transgene-free iPSCs for studying disease mechanisms and cell replacement therapies.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Cre Recombinase ; Ips Cells ; Phic31 Integrase ; Pluripotency ; Reprogramming; Pluripotent Stem-cells; Site-specific Integration; Somatic-cells; Mammalian-cells; Dopaminergic-neurons; Human Fibroblasts; Phic31 Integrase; Phage Integrase; Gene-expression; Generation
Sprache englisch
Veröffentlichungsjahr 2014
HGF-Berichtsjahr 2014
ISSN (print) / ISBN 1073-6085
e-ISSN 1559-0305
Zeitschrift Molecular Biotechnology : MB Online
Quellenangaben Band: 56, Heft: 8, Seiten: 697-713 Artikelnummer: , Supplement: ,
Verlag Springer
Verlagsort Totowa
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Developmental Genetics (IDG)
Institute of Experimental Genetics (IEG)
Institute of Stem Cell Research (ISF)
POF Topic(s) 30204 - Cell Programming and Repair
30201 - Metabolic Health
30504 - Mechanisms of Genetic and Environmental Influences on Health and Disease
Forschungsfeld(er) Genetics and Epidemiology
Stem Cell and Neuroscience
PSP-Element(e) G-500500-001
G-500600-004
G-552400-001
PubMed ID 24677035
DOI 10.1007/s12033-014-9748-y
WOS ID WOS:000339869800003
Scopus ID 84904856606
Scopus ID 84896590720
Erfassungsdatum 2014-03-30
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