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Velikova, V.B. ; Ghirardo, A. ; Vanzo, E. ; Merl, J. ; Hauck, S.M. ; Schnitzler, J.-P.

Genetic manipulation of isoprene emissions in poplar plants remodels the chloroplast proteome.

J. Proteome Res. 13, 2005-2018 (2014)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Biogenic isoprene (2-methyl-1,3-butadiene) improves the integrity and functionality of thylakoid membranes and scavenges reactive oxygen species (ROS) in plant tissue under stress conditions. On the basis of available physiological studies, we hypothesized that the suppression of isoprene production in the poplar plant by genetic engineering would cause changes in the chloroplast protein pattern, which in turn would compensate for changes in chloroplast functionality and overall plant performance under abiotic stress. To test this hypothesis, we used a stable isotope-coded protein-labeling technique in conjunction with polyacrylamide gel electrophoresis and liquid chromatography tandem mass spectrometry. We analyzed quantitative and qualitative changes in the chloroplast proteome of isoprene-emitting and non isoprene-emitting poplars. Here we demonstrate that suppression of isoprene synthase by RNA interference resulted in decreased levels of chloroplast proteins involved in photosynthesis and increased levels of histones, ribosomal proteins, and proteins related to metabolism. Overall, our results show that the absence of isoprene triggers a rearrangement of the chloroplast protein profile to minimize the negative stress effects resulting from the absence of isoprene. The present data strongly support the idea that isoprene improves/stabilizes thylakoid membrane structure and interferes with the production of ROS.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Abiotic Stress ; Defense ; Photosynthesis ; Proteomics ; Psi ; Psii ; Volatile Organic Compounds; Photosystem-ii; Oxidative Stress; Serine Hydroxymethyltransferase; Arabidopsis-thaliana; Lipoprotein Particles; Signal-transduction; Lipid-peroxidation; Plastid Proteomics; Carbon Metabolism; Emitting Poplars
Sprache englisch
Veröffentlichungsjahr 2014
HGF-Berichtsjahr 2014
ISSN (print) / ISBN 1535-3893
e-ISSN 1535-3907
Zeitschrift Journal of Proteome Research
Quellenangaben Band: 13, Heft: 4, Seiten: 2005-2018 Artikelnummer: , Supplement: ,
Verlag American Chemical Society (ACS)
Verlagsort Washington
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Environmental Simulation (EUS)
Institute of Biochemical Plant Pathology (BIOP)
CF Metabolomics & Proteomics (CF-MPC)
POF Topic(s) 30202 - Environmental Health
20402 - Sustainable Plant Production
30203 - Molecular Targets and Therapies
Forschungsfeld(er) Environmental Sciences
Enabling and Novel Technologies
PSP-Element(e) G-504991-001
G-504900-003
G-505700-001
PubMed ID 24650239
DOI 10.1021/pr401124z
WOS ID WOS:000334016400021
Scopus ID 84898743010
Erfassungsdatum 2014-05-03
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