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Arakawa, H. ; Saribasak, H. ; Buerstedde, J.-M.

Activation-induced cytidine deaminase initiates immunoglobulin gene conversion and hypermutation by a common intermediate.

PLoS Biol. 2, 967-974:e179 (2004)
Verlagsversion Volltext DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Depending on the species and the lymphoid organ, activation-induced cytidine deaminase (AID) expression triggers diversification of the rearranged immunoglobulin (Ig) genes by pseudo V (psiV) gene- templated gene conversion or somatic hypermutation. To investigate how AID can alternatively induce recombination or hypermutation, psiV gene deletions were introduced into the rearranged light chain locus of the DT40 B-cell line. We show that the stepwise removal of the psiV donors not only reduces and eventually abolishes Ig gene conversion, but also activates AID-dependent Ig hypermutation. This strongly supports a model in which AID induces a common modification in the rearranged V(D)J segment, leading to a conversion tract in the presence of nearby donor sequences and to a point mutation in their absence.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter CLASS SWITCH RECOMBINATION; DT40 CELL-LINE; B-CELLS; SOMATIC HYPERMUTATION; GERMINAL-CENTERS; AID; REQUIREMENT; REPERTOIRE; MUTANT; ENZYME
ISSN (print) / ISBN 1544-9173
e-ISSN 1545-7885
Zeitschrift PLoS Biology
Quellenangaben Band: 2, Heft: 7, Seiten: 967-974, Artikelnummer: e179 Supplement: ,
Verlag Public Library of Science (PLoS)
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Molecular Radiation Biology (IMS)