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Bartsch, R. ; Klein, D.A. ; Summer, K.H.

The Cd-Chelex assay: A new sensitive method to determine metallothionein containing zinc and cadmium.

Arch. Toxicol. 64, 177-180 (1990)

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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.

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A rapid and sensitive one-vial procedure to determine metallothionein (MT) containing zinc (Zn) and cadmium (Cd) is described. New features of this Cd-saturation method are: high mnolecular weight Cd-binding proteins are denatured by treatment with acetonitrile (50% final concentration), and excess of Cd is bound to a cation exchange resin (Chelex-100). With this method, MT has been measured, e.g. in liver of control and zinc- or cadmium-treated rats, in human liver and in cultured human fibroblasts down to absolute amounts of 0.1 μg. The Cd-Chelex assay is 10 times more sensitive than the established Cd-heme assay (Dieter et al. 1986) and therefore is particularly suitable to quantify MT in small tissue samples (e.g., liver biopsies of a few milligrams) and in extrahepatic tissues or cell cultures with low MT concentrations.

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