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Perspectives for sensitivity enhancement in proton-detected solid-state NMR of highly deuterated proteins by preserving water magnetization.
J. Biomol. NMR 61, 151-160 (2015)
In this work, we show how the water flip-back approach that is widely employed in solution-state NMR can be adapted to proton-detected MAS solid-state NMR of highly deuterated proteins. The scheme allows to enhance the sensitivity of the experiment by decreasing the recovery time of the proton longitudinal magnetization. The method relies on polarization transfer from non-saturated water to the protein during the inter-scan delay.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Times Cited
Scopus
Cited By
Cited By
Altmetric
3.141
0.894
1
2
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Magic Angle Spinning (mas) Solid-state Nmr ; Perdeuteration ; Protein Water Interaction ; Sensitivity Enchancement; Angle-spinning Nmr; Resonance Assignment; Cross-polarization; Hsqc Spectra; Flip-back; Spectroscopy; Resolution; Relaxation; Excitation; Dynamics
Sprache
englisch
Veröffentlichungsjahr
2015
HGF-Berichtsjahr
2015
ISSN (print) / ISBN
0925-2738
e-ISSN
1573-5001
Zeitschrift
Journal of Biomolecular NMR
Quellenangaben
Band: 61,
Heft: 2,
Seiten: 151-160
Verlag
Springer
Verlagsort
Dordrecht
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Structural Biology (STB)
POF Topic(s)
30203 - Molecular Targets and Therapies
Forschungsfeld(er)
Enabling and Novel Technologies
PSP-Element(e)
G-503090-001
WOS ID
WOS:000349888000007
Scopus ID
84921885416
PubMed ID
25634300
Erfassungsdatum
2015-02-06