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Stiel, A.C.* ; Dean-Ben, X.L. ; Jiang, Y. ; Ntziachristos, V. ; Razansky, D. ; Westmeyer, G.G.

High-contrast imaging of reversibly switchable fluorescent proteins via temporally unmixed multispectral optoacoustic tomography.

Opt. Lett. 40, 367-370 (2015)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Photocontrol of reversibly switchable fluorescent proteins (RSFPs) was used to program optoacoustic signal time courses that were temporally unmixed to increase the proteins' contrast-to-noise-ratios (CNRs) in optoacoustic imaging. In this way, two variants of the RSFP Dronpa with very similar optoacoustic spectra could be readily discriminated in the presence of highly absorbing blood. Addition of temporal unmixing to multispectral optoacoustic tomography (tuMSOT) in conjunction with synthetic or genetically encoded photochromic contrast agents and customized photoswitching schedules can increase the performance of multiplexed and high-contrast molecular optoacoustic imaging.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Vertex Component Analysis; Stimulated-emission; Microscopy; Gfp; Dronpa; Cells; Limit
ISSN (print) / ISBN 0146-9592
e-ISSN 1539-4794
Zeitschrift Optics Letters
Quellenangaben Band: 40, Heft: 3, Seiten: 367-370 Artikelnummer: , Supplement: ,
Verlag Optical Society of America (OSA)
Verlagsort Washington
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)
Institute of Developmental Genetics (IDG)