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High-contrast imaging of reversibly switchable fluorescent proteins via temporally unmixed multispectral optoacoustic tomography.
Opt. Lett. 40, 367-370 (2015)
Photocontrol of reversibly switchable fluorescent proteins (RSFPs) was used to program optoacoustic signal time courses that were temporally unmixed to increase the proteins' contrast-to-noise-ratios (CNRs) in optoacoustic imaging. In this way, two variants of the RSFP Dronpa with very similar optoacoustic spectra could be readily discriminated in the presence of highly absorbing blood. Addition of temporal unmixing to multispectral optoacoustic tomography (tuMSOT) in conjunction with synthetic or genetically encoded photochromic contrast agents and customized photoswitching schedules can increase the performance of multiplexed and high-contrast molecular optoacoustic imaging.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Times Cited
Scopus
Cited By
Cited By
Altmetric
2.110
2.110
36
48
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Vertex Component Analysis; Stimulated-emission; Microscopy; Gfp; Dronpa; Cells; Limit
Sprache
englisch
Veröffentlichungsjahr
2015
HGF-Berichtsjahr
2015
ISSN (print) / ISBN
0146-9592
e-ISSN
1539-4794
Zeitschrift
Optics Letters
Quellenangaben
Band: 40,
Heft: 3,
Seiten: 367-370
Verlag
Optical Society of America (OSA)
Verlagsort
Washington
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Biological and Medical Imaging (IBMI)
Institute of Developmental Genetics (IDG)
Institute of Developmental Genetics (IDG)
POF Topic(s)
30505 - New Technologies for Biomedical Discoveries
30502 - Diabetes: Pathophysiology, Prevention and Therapy
30205 - Bioengineering and Digital Health
30204 - Cell Programming and Repair
30502 - Diabetes: Pathophysiology, Prevention and Therapy
30205 - Bioengineering and Digital Health
30204 - Cell Programming and Repair
Forschungsfeld(er)
Enabling and Novel Technologies
Genetics and Epidemiology
Genetics and Epidemiology
PSP-Element(e)
G-552000-001
G-552000-002
G-505500-001
G-505590-001
G-500500-001
G-552000-002
G-505500-001
G-505590-001
G-500500-001
PubMed ID
25680049
WOS ID
WOS:000349162000019
Erfassungsdatum
2015-02-18