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Lenoir, M.* ; Grzybek, M. ; Majkowski, M.* ; Rajesh, S.* ; Kaur, J.* ; Whittaker, S.B.* ; Coskun, Ü. ; Overduin, M.*

Structural basis of dynamic membrane recognition by trans-Golgi network specific FAPP proteins.

J. Mol. Biol. 427, 966-981 (2015)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Glycosphingolipid metabolism relies on selective recruitment of the pleckstrin homology (PH) domains of FAPP proteins to the trans-Golgi network. The mechanism involved is unclear but requires recognition of phosphatidylinositol-4-phosphate (PI4P) within the Golgi membrane. We investigated the molecular basis of FAPP1-PH domain interactions with PI4P bilayers in liposome sedimentation and membrane partitioning assays. Our data reveals a mechanism in which FAPP-PH proteins preferentially target PI4P-containing liquid disordered membranes, while liquid ordered membranes were disfavored. Additionally, NMR spectroscopy was used to identify the binding determinants responsible for recognizing trans-Golgi network-like bicelles including phosphoinositide and neighboring lipid molecules. Membrane penetration by the FAPP1-PH domain was mediated by an exposed, conserved hydrophobic wedge next to the PI4P recognition site and ringed by a network of complementary polar residues and basic charges. Our data illuminates how insertion of a structured loop provides selectivity for sensing membrane fluidity and targeting to defined membrane zones and organelles. The determinants of this membrane sensing process are conserved across the CERT, OSBP and FAPP family. Hence, lipid gradients not only result in differential membrane ordering along the secretory pathway but also specifically localize diverse proteins through recognition of ensembles of lipid ligands in dynamic and deformable bilayers in order to promote anterograde trafficking.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Lipid Microdomains ; Membrane Trafficking ; Nuclear Magnetic Resonance Spectroscopy ; Phosphoinositide Recognition ; Pleckstrin Homology Domain; Oxysterol-binding-protein; Pleckstrin Homology Domains; Magnetic-resonance Relaxation; Model-free Approach; Phosphatidylinositol 4-phosphate; Glycosphingolipid Synthesis; Dependent Activation; Backbone Dynamics; Plasma-membrane; Lipid Rafts
Sprache englisch
Veröffentlichungsjahr 2015
HGF-Berichtsjahr 2015
ISSN (print) / ISBN 0022-2836
e-ISSN 1089-8638
Zeitschrift Journal of Molecular Biology
Quellenangaben Band: 427, Heft: 4, Seiten: 966-981 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort London
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Pancreatic Islet Research (IPI)
POF Topic(s) 90000 - German Center for Diabetes Research
Forschungsfeld(er) Helmholtz Diabetes Center
PSP-Element(e) G-502600-002
PubMed ID 25579996
DOI 10.1016/j.jmb.2014.12.023
WOS ID WOS:000350076400021
Erfassungsdatum 2015-02-26
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