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Wimmer, M. ; Alessandrini, F. ; Gilles, S.* ; Frank, U. ; Oeder, S. ; Hauser, M.* ; Ring, J.* ; Ferreira, F.* ; Ernst, D. ; Winkler, J.B. ; Schmitt-Kopplin, P. ; Ohnmacht, C.* ; Behrendt, H. ; Schmidt-Weber, C.B. ; Traidl-Hoffmann, C.* ; Gutermuth, J.

Pollen-derived adenosine is a necessary co-factor for ragweed allergy.

Allergy 70, 944-954 (2015)
Postprint DOI PMC
Open Access Green
BACKGROUND: Ragweed (Ambrosia artemisiifolia) is a strong elicitor of allergic airway inflammation with worldwide increasing prevalence. Various components of ragweed pollen are thought to play a role in the development of allergic responses. Aim of the study was to identify critical factors for allergenicity of ragweed pollen in a physiologic model of allergic airway inflammation METHODS: Aqueous ragweed pollen extract, the low molecular weight fraction or the major allergen Amb a 1 were instilled intranasally on 1 - 11 consecutive days and allergic airway inflammation was evaluated by bronchoalveolar lavage, lung histology, serology, gene-expression in lung tissue and measurement of lung function. Pollen-derived adenosine was removed from the extract enzymatically in order to analyze its role in ragweed-induced allergy. Migration of human neutrophils and eosinophils towards supernatants of ragweed-stimulated bronchial epithelial cells was analyzed RESULTS: Instillation of ragweed pollen extract, but not of the major allergen or the low molecular weight fraction, induced specific IgG1 , pulmonary infiltration with inflammatory cells, a Th2-associated cytokine signature in pulmonary tissue and impaired lung function. Adenosine aggravated ragweed-induced allergic lung inflammation. In vitro, human neutrophils and eosinophils migrated towards supernatants of bronchial epithelial cells stimulated with ragweed extract only if adenosine was present CONCLUSIONS: Pollen-derived adenosine is a critical factor in ragweed-pollen induced allergic airway inflammation. Future studies aim at therapeutic strategies to control these allergen-independent pathways.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Adenosine ; Adjuvant ; Allergic Inflammation ; Ragweed ; Sensitization; Airway Inflammation; Lung Inflammation; Immune-responses; Mouse Model; Pulmonary Inflammation; Lipid Mediators; V 1; Receptor; Asthma; Mice
ISSN (print) / ISBN 0105-4538
e-ISSN 1398-9995
Zeitschrift Allergy
Quellenangaben Band: 70, Heft: 8, Seiten: 944-954 Artikelnummer: , Supplement: ,
Verlag Wiley
Verlagsort Hoboken
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute for Allergy Research (IAF)
Institute of Biochemical Plant Pathology (BIOP)
Research Unit Analytical BioGeoChemistry (BGC)
Research Unit Environmental Simulation (BIOP-EUS)