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Velikova, V.B. ; Müller, C. ; Ghirardo, A. ; Rock, T. ; Aichler, M. ; Walch, A.K. ; Schmitt-Kopplin, P. ; Schnitzler, J.-P.

Knocking down of isoprene emission modifies the lipid matrix of thylakoid membranes and influences the chloroplast ultrastructure in poplar.

Plant Physiol. 168, 859-870 (2015)
Postprint DOI PMC
Open Access Green
Isoprene is a small lipophilic molecule with important functions in plant protection against abiotic stresses by improving membrane structure and scavenging reactive oxygen species. Here, we studied the lipid composition of thylakoid membranes and chloroplast ultrastructure in isoprene emitting (IE) and non-isoprene emitting (NE) poplars. We demonstrated that the total amount of mono- (MGDG), di-galactosyldiacylglycerols (DGDG), phospholipids (PL), and fatty acids is reduced in chloroplasts when isoprene biosynthesis is blocked. A significantly lower amount of unsaturated fatty acids, particularly linolenic acid (18:3) in NE chloroplasts was associated with the reduced fluidity of thylakoid membranes, which in turn negatively affects PSII photochemical efficiency (ΦPSII). The low ΦPSII in NE plants was negatively correlated with non-photochemical quenching (NPQ) and the energy-dependent (qE) component of NPQ. Transmission electron microscopy (TEM) revealed alterations in the chloroplast ultrastructure in NE compared with IE plants. NE chloroplasts were more rounded and contained less grana stacks and longer stroma thylakoids, more plastoglobules, and larger associative zones between chloroplasts and mitochondria. These results strongly support the idea that in isoprene-emitting species, the function of this molecule is closely associated with the structural organization and functioning of plastidic membranes.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Photosynthetic Electron-transport; Photosystem-ii Antenna; Chlorophyll Fluorescence; Digalactosyl-diacylglycerol; Lipoprotein Particles; Arabidopsis-thaliana; Phosphatidic-acid; Mass-spectrometry; Emitting Poplars; Higher-plants
ISSN (print) / ISBN 0032-0889
e-ISSN 1532-2548
Zeitschrift Plant Physiology
Quellenangaben Band: 168, Heft: 3, Seiten: 859-870 Artikelnummer: , Supplement: ,
Verlag American Society of Plant Biologists (ASPB)
Verlagsort Rockville
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Environmental Simulation (BIOP-EUS)
Research Unit Analytical BioGeoChemistry (BGC)
Institute of Biochemical Plant Pathology (BIOP)
Institute of Pathology (PATH)
Research Unit Analytical Pathology (AAP)