PuSH - Publikationsserver des Helmholtz Zentrums München: The <em>Caenorhabditis elegans</em> lipidome A primer for lipid analysis in <em>Caenorhabditis elegans</em>.

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Witting, M. ; Schmitt-Kopplin, P.

The Caenorhabditis elegans lipidome A primer for lipid analysis in Caenorhabditis elegans.

Arch. Biochem. Biophys. 589, 27-37 (2015)

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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.

Abstract
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Lipids play important roles in biology, ranging from building blocks of membranes to signaling lipids. The nematode and model organism Caenorhabditis elegans has been used to explore lipid metabolism and several techniques for their analysis have been employed. These techniques include different possibilities ranging from visualization of lipid droplets, analysis of total fatty acids to analysis of complex lipids using lipidomics approaches. Lipidomics evolved from metabolomics, the latest off-spring of the "omics"-technologies and aims to characterize the lipid content of a given organism or system. Although being an extensively studied model organism, only a few applications of lipidomics to C. elegans have been reported to far, but the number is steadily increasing with more applications expected in the near future. This review gives an overview on the C. elegans lipidome, lipid classes it contains and ways to analyze them. It serves as primer for scientists interested in studying lipids in this model organism and list methods used so far and what information can be derived from them. Lastly, challenges and future (methodological) research directions, together with new methods potentially useful for C. elegans lipid research are discussed.

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