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Hybrid label-free Multiphoton and Optoacoustic Microscopy (MPOM).
Proc. SPIE 9539:953908 (2015)
Verlagsversion
DOI
Many biological applications require a simultaneous observation of different anatomical features. However, unless potentially harmful staining of the specimens is employed, individual microscopy techniques do generally not provide multi-contrast capabilities. We present a hybrid microscope integrating optoacoustic microscopy and multiphoton microscopy, including second-harmonic generation, into a single device. This combined multiphoton and optoacoustic microscope (MPOM) offers visualization of a broad range of structures by employing different contrast mechanisms and at the same time enables pure label-free imaging of biological systems. We investigate the relative performance of the two microscopy modalities and demonstrate their multi-contrast abilities through the label-free imaging of a zebrafish larva ex vivo, simultaneously visualizing muscles and pigments. This hybrid microscopy application bears great potential for developmental biology studies, enabling more comprehensive information to be obtained from biological specimens without the necessity of staining.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Label-free Imaging ; Microscopy ; Multimodal Imaging ; Nonlinear Microscopy ; Photoacoustic Imaging
ISSN (print) / ISBN
0277-786X
e-ISSN
1996-756X
Konferenztitel
Opto-Acoustic Methods and Applications in Biophotonics II
Konferzenzdatum
22-23 June 2015
Konferenzort
Munich, Germany
Zeitschrift
Proceedings of SPIE
Quellenangaben
Band: 9539,
Artikelnummer: 953908
Verlag
SPIE
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed