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Filipczyk, A. ; Marr, C. ; Hastreiter, S. ; Feigelman, J. ; Schwarzfischer, M. ; Hoppe, P.S. ; Loeffler, D. ; Kokkaliaris, K.D. ; Endele, M. ; Schauberger, B. ; Hilsenbeck, O. ; Skylaki, S. ; Hasenauer, J. ; Anastassiadis, K.* ; Theis, F.J. ; Schroeder, T.

Network plasticity of pluripotency transcription factors in embryonic stem cells.

Nat. Cell Biol. 17, 1235-1246 (2015)
Anhang DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Transcription factor (TF) networks are thought to regulate embryonic stem cell (ESC) pluripotency. However, TF expression dynamics and regulatory mechanisms are poorly understood. We use reporter mouse ESC lines allowing non-invasive quantification of Nanog or Oct4 protein levels and continuous long-term single-cell tracking and quantification over many generations to reveal diverse TF protein expression dynamics. For cells with low Nanog expression, we identified two distinct colony types: one re-expressed Nanog in a mosaic pattern, and the other did not re-express Nanog over many generations. Although both expressed pluripotency markers, they exhibited differences in their TF protein correlation networks and differentiation propensities. Sister cell analysis revealed that differences in Nanog levels are not necessarily accompanied by differences in the expression of other pluripotency factors. Thus, regulatory interactions of pluripotency TFs are less stringently implemented in individual self-renewing ESCs than assumed at present.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Sprache englisch
Veröffentlichungsjahr 2015
HGF-Berichtsjahr 2015
ISSN (print) / ISBN 1465-7392
e-ISSN 1476-4679
Zeitschrift Nature Cell Biology
Quellenangaben Band: 17, Heft: 10, Seiten: 1235-1246 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Stem Cell Research (ISF)
Institute of Computational Biology (ICB)
POF Topic(s) 30204 - Cell Programming and Repair
30205 - Bioengineering and Digital Health
Forschungsfeld(er) Stem Cell and Neuroscience
Enabling and Novel Technologies
PSP-Element(e) G-500800-001
G-503800-001
G-553800-001
PubMed ID 26389663
DOI 10.1038/ncb3237
WOS ID WOS:000362213500004
Scopus ID 84942981648
Scopus ID 84941905451
Erfassungsdatum 2015-09-30
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