PuSH - Publikationsserver des Helmholtz Zentrums München

Azimzadeh, O. ; Azizova, T.V.* ; Merl-Pham, J. ; Subramanian, V. ; Bakshi, M.V. ; Moseeva, M.* ; Zubkova, O.* ; Hauck, S.M. ; Anastasov, N. ; Atkinson, M.J. ; Tapio, S.

A dose-dependent perturbation in cardiac energy metabolism is linked to radiation-induced ischemic heart disease in Mayak nuclear workers.

Oncotarget 8, 9067-9078 (2017)
Verlagsversion Anhang DOI PMC
Creative Commons Lizenzvertrag
Epidemiological studies show a significant increase in ischemic heart disease (IHD) incidence associated with total external gamma-ray dose among Mayak plutonium enrichment plant workers. Our previous studies using mouse models suggest that persistent alteration of heart metabolism due to the inhibition of peroxisome proliferator-activated receptor (PPAR) alpha accompanies cardiac damage after high doses of ionising radiation. The aim of the present study was to elucidate the mechanism of radiation-induced IHD in humans. The cardiac proteome response to irradiation was analysed in Mayak workers who were exposed only to external doses of gamma rays. All participants were diagnosed during their lifetime with IHD that also was the cause of death. Label-free quantitative proteomics analysis was performed on tissue samples from the cardiac left ventricles of individuals stratified into four radiation dose groups (0 Gy, < 100 mGy, 100-500 mGy, and > 500 mGy). The groups could be separated using principal component analysis based on all proteomics features. Proteome profiling showed a dose-dependent increase in the number of downregulated mitochondrial and structural proteins. Both proteomics and immunoblotting showed decreased expression of several oxidative stress responsive proteins in the irradiated hearts. The phosphorylation of transcription factor PPAR alpha was increased in a dose-dependent manner, which is indicative of a reduction in transcriptional activity with increased radiation dose. These data suggest that chronic external radiation enhances the risk for IHD by inhibiting PPAR alpha and altering the expression of mitochondrial, structural, and antioxidant components of the heart.
Altmetric
Weitere Metriken?
[➜Einloggen]
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Ppar Alpha ; Heart Disease ; Ionising Radiation ; Mitochondrial Dysfunction ; Proteomics; Activated Receptor-alpha; Ionizing-radiation; Label-free; Mitochondrial Dysfunction; Cardiovascular-diseases; Protein Oxidation; Ppar-alpha; Tissue; Failure; Target
ISSN (print) / ISBN 1949-2553
e-ISSN 1949-2553
Zeitschrift OncoTarget
Quellenangaben Band: 8, Heft: 6, Seiten: 9067-9078 Artikelnummer: , Supplement: ,
Verlag Impact Journals LLC
Verlagsort Orchard Park
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Radiation Biology (ISB)
CF Metabolomics & Proteomics (CF-MPC)