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Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Initial characterization of human DHRS1 (SDR19C1), a member of the short chain dehydrogenase/reductase superfamily.
J. Steroid Biochem. Mol. Biol. 185, 80-89 (2019)
Many enzymes from the short-chain dehydrogenase/reductase superfamily (SDR) have already been well characterized, particularly those that participate in crucial biochemical reactions in the human body (e.g. 11 beta-hydroxysteroid dehydrogenase 1, 17 beta-hydroxysteroid dehydrogenase 1 or carbonyl reductase 1). Several other SDR enzymes are completely or almost completely uncharacterized, such as DHRS1 (also known as SDR19C1). Based on our in silico and experimental approaches, DHRS1 is described as a likely monotopic protein that interacts with the membrane of the endoplasmic reticulum. The highest expression level of DHRS1 protein was observed in human liver and adrenals. The recombinant form of DHRS1 was purified using the detergent ndodecy1-beta-D-maltoside, and DHRS1 was proven to be an NADPH-dependent reductase that is able to catalyse the in vitro reductive conversion of some steroids (estrone, androstene-3,17-dione and cortisone), as well as other endogenous substances and xenobiotics. The expression pattern and enzyme activities fit to a role in steroid and/or xenobiotic metabolism; however, more research is needed to fully clarify the exact biological function of DHRS1.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Dhrs1 ; Sdr19c1 ; Sdr Superfamily ; Steroid Hormones ; Xenobiotics; Subcellular-localization; Membrane-proteins; Lipid Droplets; Sdr; Reconstitution; Prediction; Identification; Purification; Classification; Cytoplasm
ISSN (print) / ISBN
0960-0760
e-ISSN
0960-0760
Quellenangaben
Band: 185,
Seiten: 80-89
Verlag
Elsevier
Verlagsort
The Boulevard, Langford Lane, Kidlington, Oxford Ox5 1gb, England
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Experimental Genetics (IEG)