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Rowlands, C.J.* ; Park, D.* ; Bruns, O.T. ; Piatkevich, K.D.* ; Fukumura, D.* ; Jain, R.K.* ; Bawendi, M.G.* ; Boyden, E.S.* ; So, P.T.*

Wide-field three-photon excitation in biological samples.

Light Sci. Appl. 6:e16255 (2017)

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Three-photon wide-field depth-resolved excitation is used to overcome some of the limitations in conventional point-scanning two- and three-photon microscopy. Excitation of chromophores as diverse as channelrhodopsins and quantum dots is shown, and a penetration depth of more than 700 μm into fixed scattering brain tissue is achieved, approximately twice as deep as that achieved using two-photon wide-field excitation. Compatibility with live animal experiments is confirmed by imaging the cerebral vasculature of an anesthetized mouse; a complete focal stack was obtained without any evidence of photodamage. As an additional validation of the utility of wide-field three-photon excitation, functional excitation is demonstrated by performing three-photon optogenetic stimulation of cultured mouse hippocampal neurons expressing a channelrhodopsin; action potentials could reliably be excited without causing photodamage.

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