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Open Access Gold möglich sobald Verlagsversion bei der ZB eingereicht worden ist.
Wide-field three-photon excitation in biological samples.
Light Sci. Appl. 6:e16255 (2017)
Three-photon wide-field depth-resolved excitation is used to overcome some of the limitations in conventional point-scanning two- and three-photon microscopy. Excitation of chromophores as diverse as channelrhodopsins and quantum dots is shown, and a penetration depth of more than 700 μm into fixed scattering brain tissue is achieved, approximately twice as deep as that achieved using two-photon wide-field excitation. Compatibility with live animal experiments is confirmed by imaging the cerebral vasculature of an anesthetized mouse; a complete focal stack was obtained without any evidence of photodamage. As an additional validation of the utility of wide-field three-photon excitation, functional excitation is demonstrated by performing three-photon optogenetic stimulation of cultured mouse hippocampal neurons expressing a channelrhodopsin; action potentials could reliably be excited without causing photodamage.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Biophotonics ; Multiphoton Microscopy ; Optogenetics ; Temporal Focusing ; Three-photon
ISSN (print) / ISBN
2047-7538
e-ISSN
2047-7538
Zeitschrift
Light: Science & Applications
Quellenangaben
Band: 6,
Artikelnummer: e16255
Verlag
Nature Publishing Group
Verlagsort
London
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Helmholtz Pioneer Campus (HPC)