PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Karakus, U.* ; Thamamongood, T.* ; Ciminski, K.* ; Ran, W.* ; Günther, S.C.* ; Pohl, M.O.* ; Eletto, D.* ; Jeney, C.* ; Hoffmann, D.* ; Reiche, S.* ; Schinköthe, J.* ; Ulrich, R.* ; Wiener, J. ; Hayes, M.G.B.* ; Chang, M.W.* ; Hunziker, A.* ; Yángüez, E.* ; Aydillo, T.* ; Krammer, F.* ; Oderbolz, J.* ; Meier, M. ; Oxenius, A.* ; Halenius, A.* ; Zimmer, G.* ; Benner, C.* ; Hale, B.G.* ; García-Sastre, A.* ; Beer, M.* ; Schwemmle, M.* ; Stertz, S.*

MHC class II proteins mediate cross-species entry of bat influenza viruses.

Nature 567, 109-112 (2019)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Zoonotic influenza A viruses of avian origin can cause severe disease in individuals, or even global pandemics, and thus pose a threat to human populations. Waterfowl and shorebirds are believed to be the reservoir for all influenza A viruses, but this has recently been challenged by the identification of novel influenza A viruses in bats(1,2). The major bat influenza A virus envelope glycoprotein, haemagglutinin, does not bind the canonical influenza A virus receptor, sialic acid or any other glycan(1,3,4), despite its high sequence and structural homology with conventional haemagglutinins. This functionally uncharacterized plasticity of the bat influenza A virus haemagglutinin means the tropism and zoonotic potential of these viruses has not been fully determined. Here we show, using transcriptomic profiling of susceptible versus non-susceptible cells in combination with genome-wide CRISPR-Cas9 screening, that the major histocompatibility complex class II (MHC-II) human leukocyte antigen DR isotype (HLA-DR) is an essential entry determinant for bat influenza A viruses. Genetic ablation of the HLA-DR alpha a-chain rendered cells resistant to infection by bat influenza A virus, whereas ectopic expression of the HLA-DR complex in non-susceptible cells conferred susceptibility. Expression of MHC-II from different bat species, pigs, mice or chickens also conferred susceptibility to infection. Notably, the infection of mice with bat influenza A virus resulted in robust virus replication in the upper respiratory tract, whereas mice deficient for MHC-II were resistant. Collectively, our data identify MHC-II as a crucial entry mediator for bat influenza A viruses in multiple species, which permits a broad vertebrate tropism.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Scopus
Cited By
Altmetric
43.070
9.199
44
52
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Receptor-binding; A Virus; Expression; H17n10
Sprache
Veröffentlichungsjahr 2019
HGF-Berichtsjahr 2019
ISSN (print) / ISBN 0028-0836
e-ISSN 1476-4687
Zeitschrift Nature
Quellenangaben Band: 567, Heft: 7746, Seiten: 109-112 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Verlagsort London
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Pioneer Campus (HPC)
POF Topic(s) 30201 - Metabolic Health
Forschungsfeld(er) Pioneer Campus
PSP-Element(e) G-510002-001
DOI 10.1038/s41586-019-0955-3
WOS ID WOS:000460426900051
Scopus ID 85062613906
PubMed ID 30787439
Erfassungsdatum 2019-03-15
[➜Korrektur melden]