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Omony, J. ; Nussbaumer, T. ; Gutzat, R.*

DNA methylation analysis in plants: review of computational tools and future perspectives.

Brief. Bioinform. 21, 906-918 (2020)
Verlagsversion Postprint Forschungsdaten DOI PMC
Open Access Green
Genome-wide DNA methylation studies have quickly expanded due to advances in next-generation sequencing techniques along with a wealth of computational tools to analyze the data. Most of our knowledge about DNA methylation profiles, epigenetic heritability and the function of DNA methylation in plants derives from the model species Arabidopsis thaliana. There are increasingly many studies on DNA methylation in plants-uncovering methylation profiles and explaining variations in different plant tissues. Additionally, DNA methylation comparisons of different plant tissue types and dynamics during development processes are only slowly emerging but are crucial for understanding developmental and regulatory decisions. Translating this knowledge from plant model species to commercial crops could allow the establishment of new varieties with increased stress resilience and improved yield. In this review, we provide an overview of the most commonly applied bioinformatics tools for the analysis of DNA methylation data (particularly bisulfite sequencing data). The performances of a selection of the tools are analyzed for computational time and agreement in predicted methylated sites for A. thaliana, which has a smaller genome compared to the hexaploid bread wheat. The performance of the tools was benchmarked on five plant genomes. We give examples of applications of DNA methylation data analysis in crops (with a focus on cereals) and an outlook for future developments for DNA methylation status manipulations and data integration.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Review
Korrespondenzautor
Schlagwörter Epigenomics ; Epigenetics ; Bisulfite Sequencing ; Dna Methylation ; Plants ; Differentially Methylated Regions; Transgenerational Epigenetic Inheritance; Gene-expression; Cpg Islands; Quality-control; Reveals; Regions; Sequence; Insights; Patterns; Accurate
ISSN (print) / ISBN 1467-5463
e-ISSN 1477-4054
Zeitschrift Briefings in Bioinformatics
Quellenangaben Band: 21, Heft: 3, Seiten: 906-918 Artikelnummer: , Supplement: ,
Verlag Oxford University Press
Verlagsort Great Clarendon St, Oxford Ox2 6dp, England
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Plant Genome and Systems Biology (PGSB)
Institute of Network Biology (INET)
Institute of Environmental Medicine (IEM)