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Philipp, J. ; Sievert, W.* ; Azimzadeh, O. ; von Toerne, C. ; Metzger, F. ; Posch, A. ; Hladik, D. ; Subedi, P. ; Multhoff, G.* ; Atkinson, M.J. ; Tapio, S.

Data independent acquisition mass spectrometry of irradiated mouse lung endothelial cells reveals a STAT-associated inflammatory response.

Int. J. Radiat. Biol. 96, 642-650 (2020)
Postprint DOI PMC
Open Access Green
Purpose: Pulmonary inflammation is an adverse consequence of radiation therapy in breast cancer. The aim of this study was to elucidate biological pathways leading to this pathology. Materials and methods: Lung endothelial cells were isolated 24 h after thorax-irradiation (sham or 10 Gy X-ray) from female C57Bl/6 mice and cultivated for 6 days. Results: Quantitative proteomic analysis of lung endothelial cells was done using data independent acquisition (DIA) mass spectrometry. The data were analyzed using Ingenuity Pathway Analysis and STRINGdb. In total, 4220 proteins were identified using DIA of which 60 were dysregulated in the irradiated samples (fold change >= 2.00 or <= 0.50; q-value <0.05). Several (12/40) upregulated proteins formed a cluster of inflammatory proteins with STAT1 and IRF3 as predicted upstream regulators. The several-fold increased expression of STAT1 and STAT-associated ISG15 was confirmed by immunoblotting. The expression of antioxidant proteins SOD1 and PRXD5 was downregulated suggesting radiation-induced oxidative stress. Similarly, the phosphorylated (active) forms of STING and IRF3, both members of the cGAS/STING pathway, were downregulated. Conclusions: These data suggest the involvement of JAK/STAT and cGas/STING pathways in the genesis of radiation-induced lung inflammation. These pathways may be used as novel targets for the prevention of radiation-induced lung damage.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Cgas ; Sting Pathway ; Endothelial Cell ; Ionizing Radiation ; Pulmonary Inflammation ; Stat1; Cgamp-sting Pathway; Protein Isgylation; Dna-damage; Radiation; Immunity; Expression; Degradation; Macrophages; Mechanisms; Secretion
ISSN (print) / ISBN 0955-3002
e-ISSN 1362-3095
Zeitschrift International Journal of Radiation Biology
Quellenangaben Band: 96, Heft: 5, Seiten: 642-650 Artikelnummer: , Supplement: ,
Verlag Informa Healthcare
Verlagsort 2-4 Park Square, Milton Park, Abingdon Or14 4rn, Oxon, England
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Radiation Biology (ISB)
CF Metabolomics & Proteomics (CF-MPC)