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Klena, N.* ; Le Guennec, M.* ; Tassin, A.M.* ; van den Hoek, H. ; Erdmann, P.S.* ; Schaffer, M.* ; Geimer, S.* ; Aeschlimann, G.* ; Kovacik, L.* ; Sadian, Y.* ; Goldie, K.N.* ; Stahlberg, H.* ; Engel, B.D. ; Hamel, V.* ; Guichard, P.*

Architecture of the centriole cartwheel-containing region revealed by cryo-electron tomography.

EMBO J. 39:e106246 (2020)
Postprint DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
Centrioles are evolutionarily conserved barrels of microtubule triplets that form the core of the centrosome and the base of the cilium. While the crucial role of the proximal region in centriole biogenesis has been well documented, its native architecture and evolutionary conservation remain relatively unexplored. Here, using cryo-electron tomography of centrioles from four evolutionarily distant species, we report on the architectural diversity of the centriole's proximal cartwheel-bearing region. Our work reveals that the cartwheel central hub is constructed from a stack of paired rings with cartwheel inner densities inside. In bothParameciumandChlamydomonas, the repeating structural unit of the cartwheel has a periodicity of 25 nm and consists of three ring pairs, with 6 radial spokes emanating and merging into a single bundle that connects to the microtubule triplet via the D2-rod and the pinhead. Finally, we identified that the cartwheel is indirectly connected to the A-C linker through the triplet base structure extending from the pinhead. Together, our work provides unprecedented evolutionary insights into the architecture of the centriole proximal region, which underlies centriole biogenesis.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Cartwheel ; Centriole ; Cryo-electron Tomography ; Cryo-focused Ion Beam Milling ; In Situ; 9-fold Symmetry; Proteomic Analysis; Basal Bodies; Chlamydomonas; Protein; Sas-6; Duplication; Ultrastructure; Identification; Bld10/cep135
Sprache englisch
Veröffentlichungsjahr 2020
HGF-Berichtsjahr 2020
ISSN (print) / ISBN 0261-4189
e-ISSN 1460-2075
Zeitschrift EMBO Journal, The
Quellenangaben Band: 39, Heft: 22, Seiten: , Artikelnummer: e106246 Supplement: ,
Verlag Wiley
Verlagsort Heidelberg, Germany
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Pioneer Campus (HPC)
POF Topic(s) 30203 - Molecular Targets and Therapies
Forschungsfeld(er) Pioneer Campus
PSP-Element(e) G-510008-001
Förderungen Max Plank Society
Helmholtz Zentrum Munchen
European Research Council
Swiss National Science Foundation (SNSF)
DOI 10.15252/embj.2020106246
WOS ID WOS:000571650400001
Scopus ID 85091168274
PubMed ID 32954513
Erfassungsdatum 2020-11-06
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