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Gujrati, V. ; Ntziachristos, V.

Bioengineered bacterial vesicles for optoacoustics-guided phototherapy.

Methods Enzymol. 657, 349-364 (2021)
DOI PMC
Genetically engineered bacterial outer membrane vesicles (OMVs) offer promising applications for gene therapy, immunotherapy, and vaccine delivery. Importantly, OMVs are biocompatible, biodegradable, and easy to engineer and produce on a large scale. In this chapter, we discuss the development and application of bioengineered OMVs for optoacoustics-guided phototherapy applications (theranostics). We provide detailed protocols for OMVs preparation, characterization, and in vitro and in vivo validation. The engineered OMVs carry the biopolymer melanin, which generates a strong optoacoustic (OA) signal and intense heat upon absorption of near-infrared (NIR) light, enabling optoacoustics-guided cancer diagnosis and photothermal therapy in vivo.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Review
Korrespondenzautor
Schlagwörter Bacterial Outer Membrane Vesicles ; Cancer ; Melanin ; Optoacoustics ; Phototherapy ; Theranostics; Outer-membrane Vesicles
ISSN (print) / ISBN 0076-6879
e-ISSN 0076-6879
Zeitschrift Methods in Enzymology
Quellenangaben Band: 657, Heft: , Seiten: 349-364 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort 525 B Street, Suite 1900, San Diego, Ca 92101-4495 Usa
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Biological and Medical Imaging (IBMI)
Förderungen DFG
Deutsche Forschungsgemeinschaft (DFG), Germany
European Research Council (ERC) under the European Union