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Kundu, K. ; Melsbach, A. ; Heckel, B. ; Schneidemann, S. ; Kanapathi, D. ; Marozava, S. ; Merl-Pham, J. ; Elsner, M.

Linking increased isotope fractionation at low concentrations to enzyme activity regulation: 4-Cl phenol degradation by Arthrobacter chlorophenolicus A6.

Environ. Sci. Technol. 56, 3021-3032 (2022)
Postprint DOI PMC
Open Access Green
Slow microbial degradation of organic trace chemicals ("micropollutants") has been attributed to either downregulation of enzymatic turnover or rate-limiting substrate supply at low concentrations. In previous biodegradation studies, a drastic decrease in isotope fractionation of atrazine revealed a transition from rate-limiting enzyme turnover to membrane permeation as a bottleneck when concentrations fell below the Monod constant of microbial growth. With degradation of the pollutant 4-chlorophenol (4-CP) by Arthrobacter chlorophenolicus A6, this study targeted a bacterium which adapts its enzyme activity to concentrations. Unlike with atrazine degradation, isotope fractionation of 4-CP increased at lower concentrations, from ε(C) = -1.0 ± 0.5‰ in chemostats (D = 0.090 h-1, 88 mg L-1) and ε(C) = -2.1 ± 0.5‰ in batch (c0 = 220 mg L-1) to ε(C) = -4.1 ± 0.2‰ in chemostats at 90 μg L-1. Surprisingly, fatty acid composition indicated increased cell wall permeability at high concentrations, while proteomics revealed that catabolic enzymes (CphCI and CphCII) were differentially expressed at D = 0.090 h-1. These observations support regulation on the enzyme activity level─through either a metabolic shift between catabolic pathways or decreased enzymatic turnover at low concentrations─and, hence, reveal an alternative end-member scenario for bacterial adaptation at low concentrations. Including more degrader strains into this multidisciplinary analytical approach offers the perspective to build a knowledge base on bottlenecks of bioremediation at low concentrations that considers bacterial adaptation.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Cell Wall Permeability ; Chemostat ; Enzyme Regulation ; Isotope Effect ; Limits Of Biodegradation ; Mass Transfer ; Proteomics
ISSN (print) / ISBN 0013-936X
e-ISSN 1520-5851
Zeitschrift Environmental Science & Technology
Quellenangaben Band: 56, Heft: 5, Seiten: 3021-3032 Artikelnummer: , Supplement: ,
Verlag ACS
Verlagsort Washington, DC
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Groundwater Ecology (IGOE)
CF Metabolomics & Proteomics (CF-MPC)