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Drosophila CENH3 is sufficient for centromere formation.
Science 334, 686-690 (2011)
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DOI
PMC
CENH3 is a centromere-specific histone H3 variant essential for kinetochore assembly. Despite its central role in centromere function, there has been no conclusive evidence supporting CENH3 as sufficient to determine centromere identity. To address this question, we artificially targeted Drosophila CENH3 (CENP-A/CID) as a CID-GFP-LacI fusion protein to stably integrated lac operator (lacO) arrays. This ectopic CID focus assembles a functional kinetochore and directs incorporation of CID molecules without the LacI-anchor, providing evidence for the self-propagation of the epigenetic mark. CID-GFP-LacI-bound extrachromosomal lacO plasmids can assemble kinetochore proteins and bind microtubules, resulting in their stable transmission for several cell generations even after eliminating CID-GFP-LacI. We conclude that CID is both necessary and sufficient to serve as an epigenetic centromere mark and nucleate heritable centromere function.
Impact Factor
Scopus SNIP
Web of Science
Times Cited
Times Cited
Scopus
Cited By
Cited By
Altmetric
31.377
7.624
107
195
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern
Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
De-novo kinetochore; Chromosome segregation; Budding yeast; Chromatin; Nucleosomes; DNA; Modifiers; Bypasses; Complex
Sprache
englisch
Veröffentlichungsjahr
2011
HGF-Berichtsjahr
2011
ISSN (print) / ISBN
0036-8075
e-ISSN
1095-9203
Zeitschrift
Science
Quellenangaben
Band: 334,
Heft: 6056,
Seiten: 686-690
Verlag
American Association for the Advancement of Science (AAAS)
Begutachtungsstatus
Peer reviewed
Institut(e)
CF Monoclonal Antibodies (CF-MAB)
Research Unit Gene Vector (AGV)
Research Unit Gene Vector (AGV)
POF Topic(s)
30201 - Metabolic Health
30203 - Molecular Targets and Therapies
30203 - Molecular Targets and Therapies
Forschungsfeld(er)
Helmholtz Diabetes Center
Immune Response and Infection
Immune Response and Infection
PSP-Element(e)
G-502210-001
G-501500-001
G-501500-004
G-501500-001
G-501500-004
Scopus ID
80555125093
PubMed ID
22053052
Erfassungsdatum
2011-11-22