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Kukhtevich, I. ; Rivero-Romano, M. ; Rakesh, N. ; Bheda, P. ; Chadha, Y. ; Rosales-Becerra, P.* ; Hamperl, S. ; Bureik, D. ; Dornauer, S. ; Dargemont, C.* ; Kirmizis, A.* ; Schmoller, K.M. ; Schneider, R.

Quantitative RNA imaging in single live cells reveals age-dependent asymmetric inheritance.

Cell Rep. 41:111656 (2022)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Asymmetric inheritance of cellular content through cell division plays an important role in cell viability and fitness. The dynamics of RNA segregation are so far largely unaddressed. This is partly due to a lack of approaches to follow RNAs over multiple cellular divisions. Here, we establish an approach to quantify RNA dynamics in single cells across several generations in a microfluidics device by tagging RNAs with the diSpinach aptamer. Using S. cerevisiae as a model, we quantitatively characterize intracellular RNA transport from mothers into their buds. Our results suggest that, at cytokinesis, ENO2 diSpinach RNA is preferentially distributed to daughters. This asymmetric RNA segregation depends on the lifespan regulator Sir2 and decreases with increasing replicative age of mothers but does not result from increasing cell size during aging. Overall, our approach opens more opportunities to study RNA dynamics and inheritance in live budding yeast at the single-cell level.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Aging ; Cp: Cell Biology ; Dispinach Aptamer ; Microfluidics ; Rna Aptamer ; Rna Imaging ; Rna Inheritance ; S. Cerevisiae ; Single Cells ; Yeast; Messenger-rna; Saccharomyces-cerevisiae; Damaged Proteins; Life-span; Transcription; Transport; Localization; Segregation; Senescence; Inhibitor
ISSN (print) / ISBN 2211-1247
e-ISSN 2211-1247
Zeitschrift Cell Reports
Quellenangaben Band: 41, Heft: 7, Seiten: , Artikelnummer: 111656 Supplement: ,
Verlag Cell Press
Verlagsort 50 Hampshire St, Floor 5, Cambridge, Ma 02139 Usa
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Functional Epigenetics (IFE)
Institute of Epigenetics and Stem Cells (IES)
Förderungen Republic of Cyprus through the Research & Innovation Foundation
European Regional Development Fund
French National Research Agency
Human Frontier Science Program
Helmholtz Gesellschaft
DFG