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Tian, W. ; Gonzalez Suarez, A.F. ; López-Schier, H.

Long-term in toto Imaging of Cellular Behavior during Nerve Injury and Regeneration.

Bio Protoc. 13:e4665 (2023)
DOI PMC
Open Access Green möglich sobald Postprint bei der ZB eingereicht worden ist.
Accidental wounding of the peripheral nervous system leads to acute neural dysfunction. Normally, chronic deficits are overcome because peripheral nerves naturally regenerate. However, various genetic and metabolic defects can impair their natural regenerative capacity, which may be due to neuron-extrinsic mechanisms. Therefore, characterizing the behavior of multiple cells during nerve injury and repair in vivo is a pressing need in regenerative medicine. Here, we detail a method for precise wounding of sensory axons in zebrafish, followed by high-resolution in toto long-term quantitative videomicroscopy of neurons, Schwann cells, and macrophages. This protocol can be easily adapted to study the effects of targeted genetic or metabolic disruptions in zebrafish and other suitable organisms, as well as for screening pharmacological agents with therapeutic potential.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Glia ; Macrophages ; Microscopy ; Nerve Injury ; Repair ; Zebrafish; Repair
e-ISSN 2331-8325
Zeitschrift Bio-Protocol
Quellenangaben Band: 13, Heft: 9, Seiten: , Artikelnummer: e4665 Supplement: ,
Verlag bio-protocol.org
Verlagsort Sunnyvale, CA
Nichtpatentliteratur Publikationen
Begutachtungsstatus Peer reviewed
Institut(e) Research Unit Sensory Biology and Organogenesis (SBO)
Förderungen BMBF
Helmholtz Association