PuSH - Publikationsserver des Helmholtz Zentrums München

Export: TXT Text RIS Endnote (RIS) BIB BibTeX
Zitzmann, F.D.* ; Schmidt, S.* ; Frank, R.* ; Weigel, W.* ; Meier, M. ; Jahnke, H.G.*

Microcavity well-plate for automated parallel bioelectronic analysis of 3D cell cultures.

Biosens. Bioelectron. 250:116042 (2024)
Verlagsversion DOI PMC
Open Access Hybrid
Creative Commons Lizenzvertrag
Three-dimensional (3D) in vitro cell culture models serve as valuable tools for accurately replicating cellular microenvironments found in vivo. While cell culture technologies are rapidly advancing, the availability of non-invasive, real-time, and label-free analysis methods for 3D cultures remains limited. To meet the demand for higher-throughput drug screening, there is a demanding need for analytical methods that can operate in parallel. Microelectrode systems in combination with microcavity arrays (MCAs), offer the capability of spatially resolved electrochemical impedance analysis and field potential monitoring of 3D cultures. However, the fabrication and handling of small-scale MCAs have been labour-intensive, limiting their broader application. To overcome this challenge, we have established a process for creating MCAs in a standard 96-well plate format using high-precision selective laser etching. In addition, to automate and ensure the accurate placement of 3D cultures on the MCA, we have designed and characterized a plug-in tool using SLA-3D-printing. To characterize our new 96-well plate MCA-based platform, we conducted parallel analyses of human melanoma 3D cultures and monitored the effect of cisplatin in real-time by impedance spectroscopy. In the following we demonstrate the capabilities of the MCA approach by analysing contraction rates of human pluripotent stem cell-derived cardiomyocyte aggregates in response to cardioactive compounds. In summary, our MCA system significantly expands the possibilities for label-free analysis of 3D cell and tissue cultures, offering an order of magnitude higher parallelization capacity than previous devices. This advancement greatly enhances its applicability in real-world settings, such as drug development or clinical diagnostics.
Impact Factor
Scopus SNIP
Altmetric
10.700
0.000
Tags
Anmerkungen
Besondere Publikation
Auf Hompepage verbergern

Zusatzinfos bearbeiten
Eigene Tags bearbeiten
Privat
Eigene Anmerkung bearbeiten
Privat
Auf Publikationslisten für
Homepage nicht anzeigen
Als besondere Publikation
markieren
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter 3d Cell Culture ; Contractile Human Cardiomyocyte Clusters ; Electrochemical Impedance Spectroscopy ; Field Potential Monitoring ; Microcavity Array Technology ; Selective Laser Etching; Microelectrode Array; Cardiomyocytes; Blebbistatin; Tissue; Chip; 2d
Sprache englisch
Veröffentlichungsjahr 2024
HGF-Berichtsjahr 2024
ISSN (print) / ISBN 0956-5663
e-ISSN 1873-4235
Zeitschrift Biosensors and Bioelectronics
Quellenangaben Band: 250, Heft: , Seiten: , Artikelnummer: 116042 Supplement: ,
Verlag Elsevier
Verlagsort Oxford Fulfillment Centre The Boulevard, Langford Lane, Kidlington, Oxford Ox5 1gb, Oxon, England
Begutachtungsstatus Peer reviewed
Institut(e) Helmholtz Pioneer Campus (HPC)
POF Topic(s) 30201 - Metabolic Health
Forschungsfeld(er) Pioneer Campus
PSP-Element(e) G-510002-001
Förderungen Saxon Ministry of Science and the Fine Arts (SMWK)
European Union (EFRE)
Federal Ministry for Economics Affairs and Climate Action of Germany (BMWK)
Federal Ministry of Education and Research of Germany (BMBF)
DOI 10.1016/j.bios.2024.116042
WOS ID 001170491400001
Scopus ID 85183460175
PubMed ID 38266619
Erfassungsdatum 2024-01-29
[➜Korrektur melden]