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Truong, D.J.J. ; Geilenkeuser, J. ; Wendel, S.V. ; Wilming, J.C.H. ; Armbrust, N. ; Binder, E.M.H. ; Santl, T. ; Siebenhaar, A. ; Gruber, C. ; Phlairaharn, T. ; Živanić, M. ; Westmeyer, G.G.

Exonuclease-enhanced prime editors.

Nat. Methods 21, 455-464 (2024)
Verlagsversion DOI PMC
Open Access Hybrid
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Prime editing (PE) is a powerful gene-editing technique based on targeted gRNA-templated reverse transcription and integration of the de novo synthesized single-stranded DNA. To circumvent one of the main bottlenecks of the method, the competition of the reverse-transcribed 3' flap with the original 5' flap DNA, we generated an enhanced fluorescence-activated cell sorting reporter cell line to develop an exonuclease-enhanced PE strategy ('Exo-PE') composed of an improved PE complex and an aptamer-recruited DNA-exonuclease to remove the 5' original DNA flap. Exo-PE achieved better overall editing efficacy than the reference PE2 strategy for insertions ≥30 base pairs in several endogenous loci and cell lines while maintaining the high editing precision of PE2. By enabling the precise incorporation of larger insertions, Exo-PE complements the growing palette of different PE tools and spurs additional refinements of the PE machinery.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Dna-binding; Protein; Bacteriophage-t5; Recognition; Mechanisms; Site; Rna
Sprache englisch
Veröffentlichungsjahr 2024
HGF-Berichtsjahr 2024
ISSN (print) / ISBN 1548-7091
e-ISSN 1548-7105
Zeitschrift Nature Methods
Quellenangaben Band: 21, Heft: 3, Seiten: 455-464 Artikelnummer: , Supplement: ,
Verlag Nature Publishing Group
Verlagsort New York, NY
Begutachtungsstatus Peer reviewed
Institut(e) Insitute of Synthetic Biomedicine (ISBM)
Institute of Developmental Genetics (IDG)
POF Topic(s) 30205 - Bioengineering and Digital Health
30204 - Cell Programming and Repair
Forschungsfeld(er) Enabling and Novel Technologies
Genetics and Epidemiology
PSP-Element(e) G-509300-001
G-500500-001
Förderungen
European Innovation Council (EIC Pathfinder)
European Research Council (ERC)
Free State of Bavaria under the Excellence Strategy of the Federal Government and the Laender through the ONE MUNICH project Munich Multiscale Biofabrication
Federal Ministry of Education and Research (BMBF)
DOI 10.1038/s41592-023-02162-w
WOS ID 001154978000001
Scopus ID 85183739468
PubMed ID 38302659
Erfassungsdatum 2024-04-18
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