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Staphylococcus aureus Serine protease-like protein A (SplA) induces IL-8 by keratinocytes and synergizes with IL-17A.
Cytokine 180:156634 (2024)
BACKGROUND: Serine protease-like (Spl) proteins produced by Staphylococcus (S.) aureus have been associated with allergic inflammation. However, effects of Spls on the epidermal immune response have not been investigated. OBJECTIVES: To assess the epidermal immune response to SplA, SplD and SplE dependent on differentiation of keratinocytes and a Th2 or Th17 cytokine milieu. METHODS: Human keratinocytes of healthy controls and a STAT3-hyper-IgE syndrome (STAT3-HIES) patient were cultured in different calcium concentrations in the presence of Spls and Th2 or Th17 cytokines. Keratinocyte-specific IL-8 production and concomitant migration of neutrophils were assessed. RESULTS: SplE and more significantly SplA, induced IL-8 in keratinocytes. Suprabasal-like keratinocytes showed a higher Spl-mediated IL-8 production and neutrophil migration compared to basal-like keratinocytes. Th17 cytokines amplified Spl-mediated IL-8 production, which correlated with neutrophil recruitment. Neutrophil recruitment by keratinocytes of the STAT3-HIES patient was similar to healthy control cells. CONCLUSION: S. aureus-specific Spl proteases synergized with IL-17A on human keratinocytes with respect to IL-8 release and neutrophil migration, highlighting the importance of keratinocytes and Th17 immunity in barrier function.
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Publikationstyp
Artikel: Journalartikel
Dokumenttyp
Wissenschaftlicher Artikel
Schlagwörter
Keratinocytes ; Neutrophil Migration ; S. Aureus ; Stat3-hyper Ige Syndrome (hies) ; Serine Protease-like Proteins (spls) ; Th2/th17 Immunity; Atopic-dermatitis; Skin Barrier; Extracellular Proteases; Activated Receptor-2; Stat3 Mutations; Th2 Cytokines; Jobs Syndrome; Differentiation; Expression; Sensitization
ISSN (print) / ISBN
1043-4666
e-ISSN
1096-0023
Zeitschrift
Cytokine
Quellenangaben
Band: 180,
Artikelnummer: 156634
Verlag
Elsevier
Verlagsort
Oxford [u.a.]
Nichtpatentliteratur
Publikationen
Begutachtungsstatus
Peer reviewed
Institut(e)
Institute of Environmental Medicine (IEM)
Förderungen
Helmholtz-Future topic "Immunology and Inflammation"
Job Research Foundation
German Research Foundation
Job Research Foundation
German Research Foundation