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Huang, Y.* ; Urban, C.* ; Hubel, P.* ; Stukalov, A.* ; Pichlmair, A.

Protein turnover regulation is critical for influenza A virus infection.

Cell Syst. 15, 911-929 (2024)
Verlagsversion DOI PMC
Open Access Gold (Paid Option)
Creative Commons Lizenzvertrag
The abundance of a protein is defined by its continuous synthesis and degradation, a process known as protein turnover. Here, we systematically profiled the turnover of proteins in influenza A virus (IAV)-infected cells using a pulse-chase stable isotope labeling by amino acids in cell culture (SILAC)-based approach combined with downstream statistical modeling. We identified 1,798 virus-affected proteins with turnover changes (tVAPs) out of 7,739 detected proteins (data available at pulsechase.innatelab.org). In particular, the affected proteins were involved in RNA transcription, splicing and nuclear transport, protein translation and stability, and energy metabolism. Many tVAPs appeared to be known IAV-interacting proteins that regulate virus propagation, such as KPNA6, PPP6C, and POLR2A. Notably, our analysis identified additional IAV host and restriction factors, such as the splicing factor GPKOW, that exhibit significant turnover rate changes while their total abundance is minimally affected. Overall, we show that protein turnover is a critical factor both for virus replication and antiviral defense.
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Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Korrespondenzautor
Schlagwörter Iav ; Influenza A Virus ; Innate Immunity ; Mass Spectrometry ; Psilac ; Protein Degradation ; Protein Turnover ; Proteomics ; Restriction Factors ; Systems Virology ; Virus-host Interaction
ISSN (print) / ISBN 2405-4712
e-ISSN 2405-4720
Zeitschrift Cell Systems
Quellenangaben Band: 15, Heft: 10, Seiten: 911-929 Artikelnummer: , Supplement: ,
Verlag Elsevier
Verlagsort Maryland Heights, MO
Nichtpatentliteratur Publikationen
Institut(e) Institute of Virology (VIRO)