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Protein turnover regulation is critical for influenza A virus infection.
Cell Syst. 15, 911-929 (2024)
The abundance of a protein is defined by its continuous synthesis and degradation, a process known as protein turnover. Here, we systematically profiled the turnover of proteins in influenza A virus (IAV)-infected cells using a pulse-chase stable isotope labeling by amino acids in cell culture (SILAC)-based approach combined with downstream statistical modeling. We identified 1,798 virus-affected proteins with turnover changes (tVAPs) out of 7,739 detected proteins (data available at pulsechase.innatelab.org). In particular, the affected proteins were involved in RNA transcription, splicing and nuclear transport, protein translation and stability, and energy metabolism. Many tVAPs appeared to be known IAV-interacting proteins that regulate virus propagation, such as KPNA6, PPP6C, and POLR2A. Notably, our analysis identified additional IAV host and restriction factors, such as the splicing factor GPKOW, that exhibit significant turnover rate changes while their total abundance is minimally affected. Overall, we show that protein turnover is a critical factor both for virus replication and antiviral defense.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Iav ; Influenza A Virus ; Innate Immunity ; Mass Spectrometry ; Psilac ; Protein Degradation ; Protein Turnover ; Proteomics ; Restriction Factors ; Systems Virology ; Virus-host Interaction
ISSN (print) / ISBN
2405-4712
e-ISSN
2405-4720
Journal
Cell Systems
Quellenangaben
Volume: 15,
Issue: 10,
Pages: 911-929
Publisher
Elsevier
Publishing Place
Maryland Heights, MO
Non-patent literature
Publications
Institute(s)
Institute of Virology (VIRO)