PuSH - Publikationsserver des Helmholtz Zentrums München

Liesenhoff, C.* ; Hillenmayer, M.* ; Havertz, C.* ; Geerlof, A. ; Hartmann, D.* ; Priglinger, S.G.* ; Priglinger, C.S.* ; Ohlmann, A.*

Role of endogenous galectin-3 on cell biology of immortalized retinal pigment epithelial cells in vitro.

Int. J. Mol. Sci. 26:7622 (2025)
Verlagsversion Forschungsdaten DOI PMC
Open Access Gold
Creative Commons Lizenzvertrag
Galectin-3 is a multifunctional protein that is associated with diseases of the chorioretinal interface, in which the retinal pigment epithelium (RPE) plays a central role in disease development and progression. Since galectin-3 can function extracellularly as well as intracellularly via different mechanisms, we developed an immortalized human RPE cell line (ARPE-19) with a knockdown for galectin-3 expression (ARPE-19/LGALS3+/-) using a sgRNA/Cas9 all-in-one expression vector. By Western blot analysis, a reduced galectin-3 expression of approximately 48 to 60% in heterozygous ARPE-19/LGALS3+/- cells was observed when compared to native controls. Furthermore, ARPE-19/LGALS3+/- cells displayed a flattened, elongated phenotype with decreased E-cadherin as well as enhanced N-cadherin and α-smooth muscle actin mRNA expression, indicating an epithelial-mesenchymal transition of the cells. Compared to wildtype controls, ARPE-19/LGALS3+/- cells had significantly reduced metabolic activity to 86% and a substantially decreased proliferation to 73%. Furthermore, an enhanced cell adhesion and a diminished migration of immortalized galectin-3 knockdown RPE cells was observed compared to native ARPE-19 cells. Finally, by Western blot analysis, reduced pAKT, pERK1/2, and β-catenin signaling were detected in ARPE-19/LGALS3+/- cells when compared to wildtype controls. In summary, in RPE cells, endogenous galectin-3 appears to be essential for maintaining the epithelial phenotype as well as cell biological functions such as metabolism, proliferation, or migration, effects that might be mediated via a decreased activity of the AKT, ERK1/2, and β-catenin signaling pathways.
Altmetric
Weitere Metriken?
[➜Einloggen]
Zusatzinfos bearbeiten [➜Einloggen]
Publikationstyp Artikel: Journalartikel
Dokumenttyp Wissenschaftlicher Artikel
Schlagwörter Akt Signaling ; Arpe-19 ; Emt ; Erk Signaling ; Cell Attachment ; Cell Proliferation ; Galectin-3 ; Galectin-3 Knockdown ; Retinal Pigment Epithelium Cells ; β-catenin Signaling; Mesenchymal Transition; Macular Degeneration; Identification; Migration; Invasion
ISSN (print) / ISBN 1661-6596
e-ISSN 1422-0067
Zeitschrift International Journal of Molecular Sciences
Quellenangaben Band: 26, Heft: 15, Seiten: , Artikelnummer: 7622 Supplement: ,
Verlag MDPI
Verlagsort Basel
Begutachtungsstatus Peer reviewed
Institut(e) Institute of Structural Biology (STB)
Förderungen
Deutsche Forschungsgemeinschaft