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Ford, S.J. ; Dean-Ben, X.L. ; Razansky, D.

Cardiac function and perfusion dynamics measured on a beat-by-beat basis in the live mouse using ultra-fast 4D optoacoustic imaging.

Proc. SPIE 9323:93231U (2015)
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The fast heart rate (∼7 Hz) of the mouse makes cardiac imaging and functional analysis difficult when studying mouse models of cardiovascular disease, and cannot be done truly in real-time and 3D using established imaging modalities. Optoacoustic imaging, on the other hand, provides ultra-fast imaging at up to 50 volumetric frames per second, allowing for acquisition of several frames per mouse cardiac cycle. In this study, we combined a recently-developed 3D optoacoustic imaging array with novel analytical techniques to assess cardiac function and perfusion dynamics of the mouse heart at high, 4D spatiotemporal resolution. In brief, the heart of an anesthetized mouse was imaged over a series of multiple volumetric frames. In another experiment, an intravenous bolus of indocyanine green (ICG) was injected and its distribution was subsequently imaged in the heart. Unique temporal features of the cardiac cycle and ICG distribution profiles were used to segment the heart from background and to assess cardiac function. The 3D nature of the experimental data allowed for determination of cardiac volumes at ∼7-8 frames per mouse cardiac cycle, providing important cardiac function parameters (e.g. stroke volume, ejection fraction) on a beat-by-beat basis, which has been previously unachieved by any other cardiac imaging modality. Furthermore, ICG distribution dynamics allowed for the determination of pulmonary transit time and thus additional quantitative measures of cardiovascular function. This work demonstrates the potential for optoacoustic cardiac imaging and is expected to have a major contribution toward future preclinical studies of animal models of cardiovascular health and disease.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Cardiac Imaging ; Contrast Agents ; Indocyanine Green ; Mouse Heart ; Optoacoustic Imaging ; Optoacoustics
ISSN (print) / ISBN 0277-786X
e-ISSN 1996-756X
Journal Proceedings of SPIE
Quellenangaben Volume: 9323, Issue: , Pages: , Article Number: 93231U Supplement: ,
Publisher SPIE
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Biological and Medical Imaging (IBMI)