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Floyd, B.J.* ; Wilkerson, E.M.* ; Veling, M.T.* ; Minogue, C.E.* ; Xia, C.* ; Beebe, E.T.* ; Wrobel, R.L.* ; Cho, H.* ; Kremer, L.S. ; Alston, C.L.* ; Gromek, K.A.* ; Dolan, B.K.* ; Ulbrich, A.* ; Stefely, J.A.* ; Bohl, S.L.* ; Werner, K.M.* ; Jochem, A.* ; Westphall, M.S.* ; Rensvold, J.W.* ; Taylor, R.W.* ; Prokisch, H. ; Kim, J.P.* ; Coon, J.J.* ; Pagliarini, D.J.*

Mitochondrial protein interaction mapping identifies regulators of respiratory chain function.

Mol. Cell 63, 621-632 (2016)
Publ. Version/Full Text Postprint Research data DOI PMC
Open Access Green
Mitochondria are essential for numerous cellular processes, yet hundreds of their proteins lack robust functional annotation. To reveal functions for these proteins (termed MXPs), we assessed condition-specific protein-protein interactions for 50 select MXPs using affinity enrichment mass spectrometry. Our data connect MXPs to diverse mitochondrial processes, including multiple aspects of respiratory chain function. Building upon these observations, we validated C17orf89 as a complex I (CI) assembly factor. Disruption of C17orf89 markedly reduced CI activity, and its depletion is found in an unresolved case of CI deficiency. We likewise discovered that LYRM5 interacts with and deflavinates the electron-transferring flavoprotein that shuttles electrons to coenzyme Q (CoQ). Finally, we identified a dynamic human CoQ biosynthetic complex involving multiple MXPs whose topology we map using purified components. Collectively, our data lend mechanistic insight into respiratory chain-related activities and prioritize hundreds of additional interactions for further exploration of mitochondrial protein function.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords C15orf48 ; C2orf47 ; Dhrs4; Electron-transfer Flavoprotein; Coenzyme-q Biosynthesis; Yeast Mitochondria; Assembly Factor; Complex; Mutations; Quantification; Deficiency; Disorders; Disease
ISSN (print) / ISBN 1097-2765
e-ISSN 1097-4164
Journal Molecular Cell
Quellenangaben Volume: 63, Issue: 4, Pages: 621-632 Article Number: , Supplement: ,
Publisher Elsevier
Publishing Place Cambridge
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) Institute of Human Genetics (IHG)