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Open Access Green as soon as Postprint is submitted to ZB.
A nonradioactive assay to measure production and processing of ribosomal RNA by 4sU-tagging.
Methods Mol. Biol. 1455, 121-131 (2016)
In vivo metabolic pulse labeling is a classical approach to assess production and processing of ribosomal RNA (rRNA). However, conventional labeling techniques can be indirect and require work with radioactivity. Here, we describe in detail a protocol for in vivo metabolic labeling, purification, and readout of nascent rRNA by 4-thiouridine (4sU). We propose 4sU labeling as standard nonradioactive technique for the analysis of rRNA metabolism during ribosome biogenesis.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
4-thiouridine ; Click Chemistry ; Metabolic Pulse Labeling ; Nascent Rna ; Ribosome Biogenesis ; Rrna Synthesis
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 1455,
Pages: 121-131
Publisher
Springer
Publishing Place
Berlin [u.a.]
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Functional Epigenetics (IFE)