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O'Leary, V.B. ; Hain, S. ; Maugg, D. ; Smida, J. ; Azimzadeh, O. ; Tapio, S. ; Ovsepian, S.V. ; Atkinson, M.J.

Long non-coding RNA PARTICLE bridges histone and DNA methylation.

Sci. Rep. 7:1790 (2017)
Publ. Version/Full Text Research data DOI PMC
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PARTICLE (Gene PARTICL- 'Promoter of MAT2A-Antisense RadiaTion Induced Circulating LncRNA) expression is transiently elevated following low dose irradiation typically encountered in the workplace and from natural sources. This long non-coding RNA recruits epigenetic silencers for cis-acting repression of its neighbouring Methionine adenosyltransferase 2A gene. It now emerges that PARTICLE operates as a trans-acting mediator of DNA and histone lysine methylation. Chromatin immunoprecipitation sequencing (ChIP-seq) and immunological evidence established elevated PARTICLE expression linked to increased histone 3 lysine 27 trimethylation. Live-imaging of dbroccoli-PARTICLE revealing its dynamic association with DNA methyltransferase 1 was confirmed by flow cytometry, immunoprecipitation and direct competitive binding interaction through electrophoretic mobility shift assay. Acting as a regulatory docking platform, the long non-coding RNA PARTICLE serves to interlink epigenetic modification machineries and represents a compelling innovative component necessary for gene silencing on a global scale.
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Publication type Article: Journal article
Document type Scientific Article
Keywords Green Fluorescent Protein; X-chromosome Inactivation; Chromatin; Expression; Promoter; Genes; Maps
Language english
Publication Year 2017
HGF-reported in Year 2017
ISSN (print) / ISBN 2045-2322
e-ISSN 2045-2322
Journal Scientific Reports
Quellenangaben Volume: 7, Issue: 1, Pages: , Article Number: 1790 Supplement: ,
Publisher Nature Publishing Group
Publishing Place London
Reviewing status Peer reviewed
Institute(s) Institute of Radiation Biology (ISB)
Institute of Biological and Medical Imaging (IBMI)
POF-Topic(s) 30202 - Environmental Health
30205 - Bioengineering and Digital Health
Research field(s) Radiation Sciences
Enabling and Novel Technologies
PSP Element(s) G-500200-001
G-505500-001
PubMed ID 28496150
DOI 10.1038/s41598-017-01875-1
WOS ID WOS:000400959000057
Scopus ID 85019270583
Erfassungsdatum 2017-06-28
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