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Detection of the bacterial quorum-sensing signaling molecules N-Acyl-Homoserine Lactones (HSL) and N-Acyl-Homoserine (HS) with an Enzyme-Linked Immunosorbent Assay (ELISA) and via Ultrahigh-Performance Liquid Chromatography Coupled to Mass Spectrometry (UHPLC-MS).
Methods Mol. Biol. 1673, 61-72 (2018)
Quick and reliable quantitative methods requiring low amounts of sample volume are needed for the detection of N-acyl-homoserine lactones (HSL) and their degradation products N-acyl-homoserines (HS) in order to elucidate the occurrence and dynamics of these prevalent quorum-sensing molecules of Gram-negative bacteria in natural samples and laboratory model experiments. A combination of ELISA and UHPLC-MS is presented here which has proven to meet these requirements. Both methods can not only precisely detect and quantify HSLs but also their degradation products HS and thereby enable studying signaling dynamics in quorum sensing, which have been identified to play an essential role in bacterial communication.
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Publication type
Article: Journal article
Document type
Scientific Article
Keywords
Bacterial Signaling ; Elisa ; N-acyl-homoserine Lactone ; Quorum Sensing ; Uhplc-ms
ISSN (print) / ISBN
1064-3745
e-ISSN
1940-6029
Book Volume Title
Quorum Sensing
Journal
Methods in Molecular Biology
Quellenangaben
Volume: 1673,
Pages: 61-72
Publisher
Springer
Publishing Place
Berlin [u.a.]
Non-patent literature
Publications
Reviewing status
Peer reviewed
Institute(s)
Institute of Network Biology (INET)
Research Unit BioGeoChemistry and Analytics (BGC)
Research Unit BioGeoChemistry and Analytics (BGC)