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Nagler, J. ; Schriever, S.C. ; de Angelis, M. ; Pfluger, P.T. ; Schramm, K.-W.

Comprehensive analysis of nine monoamines and metabolites in small amounts of peripheral murine (C57Bl/6J) tissues.

Biomed. Chromatogr. 32:e4151 (2018)
Postprint Research data DOI PMC
Open Access Green
Monoamines, acting as hormones and neurotransmitters, play a critical role in multiple physiological processes ranging from cognitive function and mood to sympathetic nervous system activity, fight-or-flight response and glucose homeostasis. In addition to brain and blood, monoamines are abundant in several tissues, and dysfunction in their synthesis or signaling is associated with various pathological conditions. It was our goal to develop a method to detect these compounds in peripheral murine tissues. In this study, we employed a high-performance liquid chromatography method using electrochemical detection that allows not only detection of catecholamines but also a detailed analysis of nine monoamines and metabolites in murine tissues. Simple tissue extraction procedures were optimized for muscle (gastrocnemius, extensor digitorum longus and soleus), liver, pancreas and white adipose tissue in the range of weight 10-200mg. The system allowed a limit of detection between 0.625 and 2.5pg L-1 for monoamine analytes and their metabolites, including dopamine, 3,4-dihydroxyphenylacetic acid, 3-methoxytyramine, homovanillic acid, norepinephrine, epinephrine, 3-methoxy-4-hydroxyphenylglycol, serotonin and 5-hydroxyindoleacetic acid. Typical concentrations for different monoamines and their metabolization products in these tissues are presented for C57Bl/6J mice fed a high-fat diet.
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Publication type Article: Journal article
Document type Scientific Article
Corresponding Author
Keywords Ecd ; Electrochemical Detection ; High-performance Liquid Chromatography ; Neurotransmitters ; Peripheral Tissues; Performance Liquid-chromatography; Electrochemical Detection; Catecholamine Levels; Guinea-pig; Hplc-ecd; Rat; Serotonin; Dopamine; Brain; Mice
ISSN (print) / ISBN 0269-3879
e-ISSN 1099-0801
Journal Biomedical Chromatography
Quellenangaben Volume: 32, Issue: 4, Pages: , Article Number: e4151 Supplement: ,
Publisher Wiley
Publishing Place Hoboken
Non-patent literature Publications
Reviewing status Peer reviewed
Institute(s) PPM-MEX-Molecular EXposomics (MEX)
Institute of Diabetes and Obesity (IDO)